Palmulli Roberta, Bresteau Enzo, Raposo Graça, Montagnac Guillaume, van Niel Guillaume
Institut Curie, PSL Research University, CNRS, UMR144, Structure and Membrane Compartments, 75005 Paris, France.
Inserm U1279, Gustave Roussy Institute, Université Paris-Saclay, 94805 Villejuif, France.
Int J Mol Sci. 2023 Feb 12;24(4):3703. doi: 10.3390/ijms24043703.
Extracellular vesicles are now considered as active contributors to melanoma progression through their capacity to modify the tumor microenvironment and to favor the formation of a pre-metastatic niche. These prometastatic roles of tumor-derived EVs would pass through their interaction with the extracellular matrix (ECM) and its remodeling, in turn providing a substrate favoring persistent tumor cell migration. Nevertheless, the capacity of EVs to directly interact with ECM components is still questionable. In this study, we use electron microscopy and a pull-down assay to test the capacity of sEVs, derived from different melanoma cell lines, to physically interact with collagen I. We were able to generate collagen fibrils coated with sEVs and to show that melanoma cells release subpopulations of sEVs that can differentially interact with collagen.
细胞外囊泡现在被认为是黑色素瘤进展的积极贡献者，因为它们有能力改变肿瘤微环境并促进前转移小生境的形成。肿瘤衍生的细胞外囊泡的这些促转移作用将通过它们与细胞外基质（ECM）的相互作用及其重塑来实现，进而提供有利于肿瘤细胞持续迁移的底物。然而，细胞外囊泡直接与细胞外基质成分相互作用的能力仍然存在疑问。在本研究中，我们使用电子显微镜和下拉试验来测试源自不同黑色素瘤细胞系的小细胞外囊泡（sEVs）与I型胶原物理相互作用的能力。我们能够生成包被有sEVs的胶原纤维，并表明黑色素瘤细胞释放的sEVs亚群能够与胶原发生不同的相互作用。
