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过量表达 ，一种来自 的转录因子，增加烟草对干旱和盐胁迫的耐受性。

Overexpression of , a Transcription Factor from , Increases Tolerance to Drought and Salt Stress in Tobacco.

机构信息

Department of Biological Sciences, Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo, Piracicaba 13418-900, Brazil.

Department of Plant Nutrition, Centro de Edafología Aplicada del Segura (CEBAS)-CSIC, 30100 Murcia, Spain.

出版信息

Int J Mol Sci. 2023 Feb 19;24(4):4149. doi: 10.3390/ijms24044149.

DOI:10.3390/ijms24044149
PMID:36835560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9961280/
Abstract

Teak () is one of the most important wood sources, and it is cultivated in tropical regions with a significant market around the world. Abiotic stresses are an increasingly common and worrying environmental phenomenon because it causes production losses in both agriculture and forestry. Plants adapt to these stress conditions by activation or repression of specific genes, and they synthesize numerous stress proteins to maintain their cellular function. For example, APETALA2/ethylene response factor (AP2/ERF) was found to be involved in stress signal transduction. A search in the teak transcriptome database identified an gene named with a key AP2/ERF domain. We then verified that the expression is rapidly induced by Polyethylene Glycol (PEG), NaCl, and exogenous phytohormone treatments, suggesting a potential role in drought and salt stress tolerance in teak. The full-length coding sequence of gene was isolated from teak young stems, characterized, cloned, and constitutively overexpressed in tobacco plants. In transgenic tobacco plants, the overexpressed TgERF1 protein was localized exclusively in the cell nucleus, as expected for a transcription factor. Furthermore, functional characterization of provided evidence that is a promising candidate gene to be used as selective marker on plant breeding intending to improve plant stress tolerance.

摘要

柚木是最重要的木材来源之一，它生长在热带地区，在全球范围内有很大的市场。非生物胁迫是一种日益普遍和令人担忧的环境现象，因为它会导致农业和林业的产量损失。植物通过激活或抑制特定基因来适应这些胁迫条件，并合成许多应激蛋白来维持其细胞功能。例如，APETALA2/乙烯响应因子（AP2/ERF）被发现参与胁迫信号转导。在柚木转录组数据库中搜索，鉴定出一个名为 的基因，该基因具有关键的 AP2/ERF 结构域。我们随后验证了该基因的表达可被聚乙二醇（PEG）、NaCl 和外源植物激素处理迅速诱导，表明其在柚木的干旱和耐盐性中可能发挥作用。该基因的全长编码序列从柚木幼茎中分离出来，进行了特征描述、克隆，并在烟草植物中组成型过表达。在转基因烟草植物中，过表达的 TgERF1 蛋白仅定位于细胞核内，符合转录因子的预期定位。此外，对 进行功能表征的结果提供了证据，表明 是一个很有前途的候选基因，可作为植物育种中的选择标记，旨在提高植物的胁迫耐受性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/1d0e484f08c6/ijms-24-04149-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/1d0e484f08c6/ijms-24-04149-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/573dbea51777/ijms-24-04149-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/b5356223fb92/ijms-24-04149-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/6cb114ef19bf/ijms-24-04149-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/5fd86e6d8d1b/ijms-24-04149-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/c5036497a754/ijms-24-04149-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/111cddd0772b/ijms-24-04149-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/5648d21536b5/ijms-24-04149-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/ca54f78195dd/ijms-24-04149-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/a1db6758cea2/ijms-24-04149-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/4c019b047447/ijms-24-04149-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/bce09f6fff06/ijms-24-04149-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4522/9961280/1d0e484f08c6/ijms-24-04149-g013.jpg

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