在中诱导免疫反应的枯草杆菌蛋白酶样丝氨酸蛋白酶 CJPRB 的作用。

Role of the Subtilisin-like Serine Protease CJPRB from in Eliciting an Immune Response in .

机构信息

Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Int J Mol Sci. 2023 Feb 20;24(4):4170. doi: 10.3390/ijms24044170.

is a globally distributed quarantine plant pest. In a previous study, the strain BE01 with a strong pathogenic effect on was identified, and overexpression of the subtilisin-like serine protease CJPRB of this strain was found to accelerate the death of (previous research results). In this study, the active recombinant CJPRB protein was obtained through the expression system. It was found that CJPRB protein administration to via infectation, feeding and injection was able to induce changes in protective enzymes, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and polyphenol oxidase (PPO), and the expression of immune defense-related genes in . In particular, CJPRB protein injection induced a more rapid, widespread and intense immune response in compared to the other two treatment methods. The results suggest that the CJPRB protein may play a role in eliciting a host immune response during infectation by .

是一种全球分布的检疫性植物病原菌。在之前的研究中，鉴定出对具有强烈致病作用的 BE01 菌株，并且发现该菌株的枯草杆菌蛋白酶样丝氨酸蛋白酶 CJPRB 的过表达加速了的死亡（先前的研究结果）。在本研究中，通过表达系统获得了活性重组 CJPRB 蛋白。研究发现，通过侵染、喂食和注射将 CJPRB 蛋白施用于，能够诱导保护酶的变化，包括超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和多酚氧化酶（PPO），以及中免疫防御相关基因的表达。特别是，与另外两种处理方法相比，CJPRB 蛋白注射在中诱导了更快、更广泛和更强烈的免疫反应。结果表明，在侵染过程中，CJPRB 蛋白可能在引发宿主免疫反应中发挥作用。