Zhang Zhiwei, Xiong Qunli, Xu Yongfeng, Cai Xuebin, Zhang Lisha, Zhu Qing
Abdominal Oncology Ward, Cancer Center, West China Hospital of Sichuan University, Chengdu 610041, China.
J Clin Med. 2023 Feb 9;12(4):1398. doi: 10.3390/jcm12041398.
The tumor microenvironment (TME) plays a vital role in the development, progression, and metastasis of pancreatic cancer (PC). The composition of the TME and its potential prognostic value remains to be fully understood, especially in adenosquamous carcinoma of pancreas (ASCP) patients. Immunohistochemistry was used to explore the clinical significance of CD3, CD4, CD8, FoxP3, and PD-L1 expression within the TME and to identify correlations with the prognosis of PC in a series of 29 patients with ASCP and 54 patients with pancreatic ductal adenocarcinoma (PDAC). Data from the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) were accessed to obtain the scRNA-seq data and transcriptome profiles. Seurat was used to process the scRNA-seq data, and CellChat was used to analyze cell-cell communication. CIBERSORT was used to approximate the constitution of tumor-infiltrating immune cell (TICs) profiles. Higher levels of PD-L1 were linked with a shorter overall survival in ASCP ( = 0.0007) and PDAC ( = 0.0594). A higher expression of CD3+ and CD8+ T-cell infiltration was significantly correlated with a better prognosis in PC. By influencing the composition of tumor-infiltrating immune cells (TICs), high levels of PD-L1 expression are linked with a shorter overall survival in ASCP and PDAC.
肿瘤微环境（TME）在胰腺癌（PC）的发生、发展和转移中起着至关重要的作用。TME的组成及其潜在的预后价值仍有待充分了解，尤其是在胰腺腺鳞癌（ASCP）患者中。采用免疫组织化学方法探讨29例ASCP患者和54例胰腺导管腺癌（PDAC）患者TME中CD3、CD4、CD8、FoxP3和PD-L1表达的临床意义，并确定其与PC预后的相关性。从基因表达综合数据库（GEO）和癌症基因组图谱（TCGA）获取数据，以获得单细胞RNA测序（scRNA-seq）数据和转录组图谱。使用Seurat处理scRNA-seq数据，使用CellChat分析细胞间通讯。使用CIBERSORT估算肿瘤浸润免疫细胞（TICs）图谱的组成。在ASCP（P = 0.0007）和PDAC（P = 0.0594）中，较高水平的PD-L1与较短的总生存期相关。CD3+和CD8+ T细胞浸润的高表达与PC的较好预后显著相关。通过影响肿瘤浸润免疫细胞（TICs）的组成，高水平的PD-L1表达与ASCP和PDAC较短的总生存期相关。
