干燥综合征患者口腔微生物群的高通量测序：一项初步横断面研究。
High-Throughput Sequencing of Oral Microbiota in Carriage Sjögren's Syndrome Patients: A Pilot Cross-Sectional Study.
作者信息
Xing Haixia, Liu Hongwei, Pan Jie
机构信息
Department of General Dentistry, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Department of Oral Medicine, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University School and Hospital of Stomatology, Beijing 100081, China.
出版信息
J Clin Med. 2023 Feb 16;12(4):1559. doi: 10.3390/jcm12041559.
BACKGROUND
This study sought to characterize the saliva microbiota of carriage Sjögren's syndrome (SS) patients compared to oral candidiasis and healthy patients by high-throughput sequencing.
METHODS
Fifteen patients were included, with five carriage SS patients (decayed, missing, and filled teeth (DMFT) score 22), five oral candidiasis patients (DMFT score 17), and five caries active healthy patients (DMFT score 14). Bacterial 16S rRNA was extracted from rinsed whole saliva. PCR amplification generated DNA amplicons of the V3-V4 hypervariable region, which were sequenced on an Illumina HiSeq 2500 sequencing platform and compared and aligned to the SILVA database. Taxonomy abundance and community structure diversity was analyzed using Mothur software v1.40.0.
RESULTS
A total of 1016/1298/1085 operational taxonomic units (OTUs) were obtained from SS patients/oral candidiasis patient/healthy patients. , , , , and were the primary genera in the three groups. The most abundant significantly mutative taxonomy (OTU001) was . Microbial diversity (alpha diversity and beta diversity) was significantly increased in SS patients. ANOSIM analyses revealed significantly different microbial compositional heterogeneity in SS patients compared to oral candidiasis and healthy patients.
CONCLUSION
Microbial dysbiosis differs significantly in SS patients independent of oral carriage and DMFT.
背景
本研究旨在通过高通量测序对携带干燥综合征（SS）的患者与口腔念珠菌病患者及健康患者的唾液微生物群进行特征分析。
方法
纳入15例患者，其中5例携带SS的患者（龋失补牙（DMFT）评分22），5例口腔念珠菌病患者（DMFT评分17），以及5例龋活跃的健康患者（DMFT评分14）。从冲洗后的全唾液中提取细菌16S rRNA。PCR扩增产生V3-V4高变区的DNA扩增子，在Illumina HiSeq 2500测序平台上进行测序，并与SILVA数据库进行比较和比对。使用Mothur软件v1.40.0分析分类丰度和群落结构多样性。
结果
从SS患者/口腔念珠菌病患者/健康患者中分别获得了1016/1298/1085个可操作分类单元（OTU）。 、 、 、 及 是三组中的主要属。最丰富的显著变异分类群（OTU001）是 。SS患者的微生物多样性（α多样性和β多样性）显著增加。ANOSIM分析显示，与口腔念珠菌病患者和健康患者相比，SS患者的微生物组成异质性显著不同。
结论
SS患者的微生物失调与口腔携带情况和DMFT无关，存在显著差异。
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