Starzyńska Anna, Sobocki Bartosz Kamil, Sakowicz-Burkiewicz Monika, Jereczek-Fossa Barbara Alicja, Alterio Daniela, Szot Olga, Korwat Aleksandra, Pęksa Rafał
Department of Oral Surgery, Medical University of Gdansk, 7 Dębinki Street, 80-211 Gdansk, Poland.
Department of Molecular Medicine, Medical University of Gdansk, 7 Dębinki Street, 80-211 Gdansk, Poland.
J Clin Med. 2023 Feb 17;12(4):1619. doi: 10.3390/jcm12041619.
Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer in the world. Despite its prevalence, it is often recognized in advanced stages (III or IV) when it has already spread to local lymph nodes. In this study, we investigate the V-domain Ig suppressor of T cell activation (VISTA) as a potential prognostic factor in OSCC. Tissue samples were collected from 71 oral squamous cell carcinoma patients to determine protein expression levels (using immunochemistry and the semi-quantitative H-score method). Moreover, RT-qPCR was additionally performed in 35 patients. Clinical factors in our cohort study had no impact on VISTA expression. However, VISTA expression is largely correlated with Il-33 levels in tumor cells and lymphocytes and with PD-L1 in tumor cells. The impact of VISTA expression on overall survival (OS) is rather limited, but in the case of a 5-year survival rate, a significant association has been proven. VISTA seems to be a rather weak clinicopathological marker but needs further evaluation in the context of survival. In addition, the potential of VISTA combination with Il-33 or PD-L1 should be further investigated in OSCC.
口腔鳞状细胞癌（OSCC）是全球最常见的口腔癌类型。尽管其发病率很高，但往往在晚期（III期或IV期）才被发现，此时癌症已经扩散到局部淋巴结。在本研究中，我们研究了T细胞活化V结构域免疫球蛋白抑制因子（VISTA）作为OSCC潜在预后因素的情况。从71例口腔鳞状细胞癌患者中收集组织样本，以确定蛋白质表达水平（采用免疫化学和半定量H评分法）。此外，还对35例患者进行了逆转录定量聚合酶链反应（RT-qPCR）。我们队列研究中的临床因素对VISTA表达没有影响。然而，VISTA表达在很大程度上与肿瘤细胞和淋巴细胞中的白细胞介素-33（Il-33）水平以及肿瘤细胞中的程序性死亡配体-1（PD-L1）相关。VISTA表达对总生存期（OS）的影响相当有限，但就5年生存率而言，已证实存在显著关联。VISTA似乎是一个相当弱的临床病理标志物，但在生存背景下需要进一步评估。此外，VISTA与Il-33或PD-L1联合应用在OSCC中的潜力应进一步研究。
