Kapeua-Ndacnou Miraine, de Abreu Lucas Magalhães, de Macedo Davi Mesquita, da Nóbrega Thaisa Ferreira, Pereira Caio Mattos, Evans Harry Charles, Barreto Robert Weingart
Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil.
Regional Biocontrol and Applied Microbiology Laboratory, Institute of Agricultural Research for Development, Yaoundé P.O. Box 2067, Cameroon.
J Fungi (Basel). 2023 Feb 13;9(2):248. doi: 10.3390/jof9020248.
During surveys conducted in South America and Africa to identify natural fungal enemies of coffee leaf rust (CLR), , over 1500 strains were isolated, either as endophytes from healthy tissues of Coffea species or as mycoparasites growing on rust pustules. Based on morphological data, eight isolates-three isolated from wild or semiwild coffee and five from species on coffee, all from Africa-were provisionally assigned to the genus . A polyphasic study of their morphological, cultural and molecular characteristics-including the Tef1 (translation elongation factor 1 alpha), RPB1 (largest subunit of RNA polymerase II), TUB (β-tubulin) and ACL1 (ATP citrate lyase) regions-confirmed these isolates as belonging to three species of the genus : namely , and . Preliminary assays were also conducted to test the potential of the isolates to reduce CLR severity on coffee under greenhouse conditions. Foliar and soil applications indicated that seven of the isolates had a significant effect ( < 0.05) in reducing CLR severity. In parallel, in vitro tests that involved conidia suspensions of each of the isolates together with urediniospores of resulted in high levels of inhibition of urediniospore germination. All eight isolates showed their ability to establish as endophytes in during this study, and some proved to be mycoparasites of . In addition to reporting the first records of associated with healthy coffee tissues and with rusts of coffee, this work provides the first evidence that isolates have potential as biological control agents against CLR.
在南美洲和非洲进行的旨在鉴定咖啡叶锈病（CLR）天然真菌天敌的调查中，分离出了1500多个菌株，这些菌株要么是从咖啡属植物健康组织中分离得到的内生菌，要么是在锈病脓疱上生长的真菌寄生物。根据形态学数据，八个分离株——三个从野生或半野生咖啡中分离得到，五个从非洲咖啡属植物上分离得到——被初步归为该属。对它们的形态、培养和分子特征（包括Tef1（翻译延伸因子1α）、RPB1（RNA聚合酶II最大亚基）、TUB（β-微管蛋白）和ACL1（ATP柠檬酸裂解酶）区域）进行的多相研究证实，这些分离株属于该属的三个物种，即[具体物种1]、[具体物种2]和[具体物种3]。还进行了初步试验，以测试这些分离株在温室条件下降低咖啡上CLR严重程度的潜力。叶面和土壤施用表明，其中七个分离株在降低CLR严重程度方面有显著效果（P<0.05）。同时，涉及每个分离株的分生孢子悬浮液与[锈菌名称]夏孢子的体外试验导致夏孢子萌发受到高度抑制。在本研究中，所有八个分离株都显示出能够在[宿主植物名称]中作为内生菌定殖，并且一些被证明是[锈菌名称]的真菌寄生物。除了报告与健康咖啡组织以及咖啡[锈病名称]相关的[真菌名称]的首次记录外，这项工作还提供了首个证据，表明[真菌名称]分离株具有作为CLR生物防治剂的潜力。
