Caraban Bogdan Marian, Matei Elena, Cozaru Georgeta Camelia, Aşchie Mariana, Deacu Mariana, Enciu Manuela, Bălţătescu Gabriela Izabela, Chisoi Anca, Dobrin Nicolae, Petcu Lucian, Gheorghe Emma, Hangan Laurențiu-Tony, Roșu Mihai Cătălin, Orasanu Cristian Ionuț, Nicolau Antonela-Anca
Faculty of Medicine, "Ovidius" University of Constanta, 900470 Constanta, Romania.
Center for Research and Development of the Morphological and Genetic Studies of Malignant Pathology, "Ovidius" University of Constanta, 900591 Constanta, Romania.
J Pers Med. 2023 Jan 27;13(2):221. doi: 10.3390/jpm13020221.
(1) Background: Because melanoma is an aggressive tumor with an unfavorable prognosis, we aimed to characterize the PD-L1 expression in melanomas in association with T cell infiltrates because PD-1/PD-L1 blockade represents the target in treating melanoma strategy. (2) Methods: The immunohistochemical manual quantitative methods of PD-L1, CD4, and CD8 TILs were performed in melanoma tumor microenvironment cells. (3) Results: Most of the PD-L1 positive, expressing tumors, have a moderate score of CD4+ TILs and CD8+TILs (5-50% of tumor area) in tumoral melanoma environment cells. The PD-L1 expression in TILs was correlated with different degrees of lymphocytic infiltration described by the Clark system (X = 8.383, = 0.020). PD-L1 expression was observed often in melanoma cases, with more than 2-4 mm of Breslow tumor thickness being the associated parameters (X = 9.933, = 0.014). (4) Conclusions: PD-L1 expression represents a predictive biomarker with very good accuracy for discriminating the presence or absence of malign tumoral melanoma cells. PD-L1 expression was an independent predictor of good prognosis in patients with melanomas.
(1) 背景：由于黑色素瘤是一种侵袭性肿瘤，预后不佳，我们旨在研究黑色素瘤中PD-L1表达与T细胞浸润的关系，因为PD-1/PD-L1阻断是治疗黑色素瘤策略的靶点。(2) 方法：采用免疫组织化学手工定量方法检测黑色素瘤肿瘤微环境细胞中的PD-L1、CD4和CD8肿瘤浸润淋巴细胞（TILs）。(3) 结果：在肿瘤性黑色素瘤环境细胞中，大多数PD-L1阳性表达肿瘤的CD4+TILs和CD8+TILs评分中等（占肿瘤面积的5-50%）。TILs中的PD-L1表达与Clark系统描述的不同程度淋巴细胞浸润相关（X = 8.383，P = 0.020）。在黑色素瘤病例中经常观察到PD-L1表达，Breslow肿瘤厚度超过2-4 mm是相关参数（X = 9.933，P = 0.014）。(4) 结论：PD-L1表达是一种预测生物标志物，在鉴别恶性肿瘤性黑色素瘤细胞的有无方面具有很高的准确性。PD-L1表达是黑色素瘤患者预后良好的独立预测因子。
