Stępień Krzysztof Adam, Krawczyk Weronika, Giebułtowicz Joanna
Department of Drugs Chemistry, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha, 02-097 Warsaw, Poland.
Life (Basel). 2023 Jan 18;13(2):263. doi: 10.3390/life13020263.
Dietary supplements are food products commonly used worldwide to obtain nutritional and physiological effects. They can contain a wide variety of active substances and can be administered for health and disease. Their use can be beneficial if justified, and their quality is adequate. Unfortunately, data on the quality of supplements is scarce. As part of this work, we assess the quality of seven dietary supplements containing proline. The preparations were produced in the EU and the USA. The quality assessment consisted of the detection of potential impurities, the determination of the content of the main ingredient, and the release of proline. The technique used to analyse impurities and proline (Pro) content was liquid chromatography coupled with tandem mass spectrometry. We detected five contaminants. The main ingredient content was in the range of 73-121% in capsules and 103-156% in tablets. Five of the seven analysed dietary supplements released below 80% Pro (for each tablet/capsule at pH 1.2). One of the supplements may be inactive because a very low release of Pro was reported. The results, we hope, will increase consumer awareness of the quality of these preparations and result in a change in the regulations governing the marketing of these preparations, at least by making release testing mandatory.
膳食补充剂是全球普遍使用的食品，用于获取营养和生理功效。它们可以包含各种各样的活性物质，可用于健康和疾病防治。如果使用合理且质量合格，其使用可能有益。不幸的是，关于补充剂质量的数据很少。作为这项工作的一部分，我们评估了七种含脯氨酸的膳食补充剂的质量。这些制剂产自欧盟和美国。质量评估包括检测潜在杂质、测定主要成分的含量以及脯氨酸的释放情况。用于分析杂质和脯氨酸（Pro）含量的技术是液相色谱-串联质谱联用。我们检测到了五种污染物。胶囊中主要成分的含量在73%-121%之间，片剂中在103%-156%之间。七种分析的膳食补充剂中有五种在pH 1.2时每片/每粒胶囊的脯氨酸释放量低于80%。其中一种补充剂可能没有活性，因为报告显示脯氨酸释放量非常低。我们希望这些结果将提高消费者对这些制剂质量的认识，并导致管理这些制剂销售的法规发生变化，至少强制进行释放测试。
