Valverde Sancho Juan, Carreño Amate Cristina, Caparrós Pérez María Del Mar, Santana Méridas Omar, Julio Luis F
Green Chemistry Area, R&D Department, Kimitec Group, Paraje Cerro de Los Lobos, 04738 Vícar, Spain.
Phytopathology Area, R&D Department, Kimitec Group, Paraje Cerro de Los Lobos, 04738 Vícar, Spain.
Life (Basel). 2023 Feb 11;13(2):499. doi: 10.3390/life13020499.
Plants have been used by humans since ancient times due their antimicrobial and medicinal properties. Essential oils (EOs) are complex mixtures of secondary plant metabolites, including terpenoids, phenylpropanoids, and other aromatic compounds. and EOs and their organic extracts exert numerous biological activities because of their major compounds, particularly thymol, carvacrol, eugenol, and benzoic and cinnamic acid. The structural motifs presented by these phytochemicals are responsible for their biological activities. Modification or hybridization of these structures could lead to new bio-based compounds with improved efficacy or multiple modes of action. In this work, we aimed to develop reliable methods of obtaining six hybrid molecules from the major constituents of and EOs. For the first time, we tested their efficacy in the inhibition of the mycelium growth and spore germination of two of the most important phytopathogenic fungi, and , and one opportunistic human pathogen, . The cytotoxic activity of the obtained hybrids was assessed using the brine shrimp lethality assay. In addition, we report for the first time a biocatalytic process for the obtention of these bioactive hybrid molecules. The results of this work enable the possibility of using hybrid molecules based on the major constituents of EOs as active ingredients in strategic industries such as agriculture, aquaculture, and pharmaceuticals.
自古以来，植物就因其抗菌和药用特性而被人类使用。精油（EOs）是植物次生代谢产物的复杂混合物，包括萜类化合物、苯丙素类化合物和其他芳香化合物。由于其主要成分，特别是百里香酚、香芹酚、丁香酚以及苯甲酸和肉桂酸，精油及其有机提取物具有多种生物活性。这些植物化学物质呈现的结构基序决定了它们的生物活性。对这些结构进行修饰或杂交可能会产生具有更高功效或多种作用方式的新型生物基化合物。在这项工作中，我们旨在开发从两种精油的主要成分中获得六种杂化分子的可靠方法。我们首次测试了它们对两种最重要的植物病原真菌以及一种机会性人类病原体的菌丝体生长和孢子萌发的抑制效果。使用卤虫致死率测定法评估所获得杂化分子的细胞毒性活性。此外，我们首次报道了获得这些生物活性杂化分子的生物催化过程。这项工作的结果使得基于精油主要成分的杂化分子有可能作为活性成分应用于农业、水产养殖和制药等战略产业。
