Department of Environmental Medical Biology, Wonju College of Medicine Yonsei University, Wonju, Gangwon 26426, Republic of Korea.
Department of Laboratory Medicine, Wonju College of Medicine Yonsei University, Wonju, Gangwon 26426, Republic of Korea.
Oxid Med Cell Longev. 2020 Dec 12;2020:2459826. doi: 10.1155/2020/2459826. eCollection 2020.
Strong acidic electrolyzed water (StAEW) is known to inactivate microorganisms but is not fully explored in the medical field. This study is aimed at exploring StAEW as a potential wound care agent and its mechanism. StAEW (pH: 2.65, ORP: 1159 mV, ACC: 32.1 ppm) was sprayed three times a day to the cutaneous wounds of hairless mice for seven days. Wound morphological and histological features and immune-redox markers were compared with saline- (Sal-) and alcohol- (Alc-) treated groups. Results showed that the StAEW group showed a significantly higher wound healing percentage than the Sal group on days 2, 4, 5, and 6 and the Alc group on day 4. The StAEW group also showed earlier mediation on proinflammatory cytokines such as tumor necrosis factor-, interleukin- (IL-) 6, IL-1, and keratinocyte chemoattractant. In addition, basic fibroblast growth factor and platelet-derived growth factor were found to be significantly changed in favor of the fibroblast synthesis and angiogenesis. In line, the StAEW group showed a controlled amount of ROS and significantly decreased compared to the Alc group. The StAEW group also favored oxidative stress balance through antioxidant responses. Additionally, matrix metalloproteinases (MMP) 9 and MMP1 were also modulated for keratinocyte and cell migration. Taken together, this study has proven the wound healing effect of StAEW and its earlier mediation through oxidative and inflammatory responses.
强酸性电解水（StAEW）已被证明可以灭活微生物，但在医学领域尚未得到充分探索。本研究旨在探索 StAEW 作为一种潜在的伤口护理剂及其作用机制。将 StAEW（pH：2.65，ORP：1159 mV，ACC：32.1 ppm）每天喷洒三次到无毛小鼠的皮肤伤口上，持续七天。比较了盐水（Sal）和酒精（Alc）处理组的伤口形态学和组织学特征以及免疫氧化还原标志物。结果表明，在第 2、4、5 和 6 天，StAEW 组的伤口愈合百分比明显高于 Sal 组，在第 4 天，StAEW 组也明显高于 Alc 组。StAEW 组还更早地介导了肿瘤坏死因子-α、白细胞介素-（IL）-6、IL-1 和角质形成细胞趋化因子等促炎细胞因子。此外，碱性成纤维细胞生长因子和血小板衍生生长因子的含量明显发生变化，有利于成纤维细胞的合成和血管生成。相应地，StAEW 组的 ROS 含量得到控制，与 Alc 组相比显著降低。StAEW 组还通过抗氧化反应有利于氧化应激平衡。此外，基质金属蛋白酶（MMP）9 和 MMP1 也被调节以促进角质形成细胞和细胞迁移。综上所述，本研究证明了 StAEW 的伤口愈合作用及其通过氧化和炎症反应的早期介导作用。
