Campolo Allison, Patterson Brian, Lara Esther, Shannon Paul, Crary Monica
Alcon Research, LLC, Fort Worth, TX 76134, USA.
Microorganisms. 2023 Jan 23;11(2):299. doi: 10.3390/microorganisms11020299.
keratitis is a sight-threatening infection of the cornea which is extremely challenging to treat. Understanding this organism's responses during contact lens contact and disinfection could enhance our understanding of how colonize contact lens cases, better inform us on contact lens care solution (CLC) efficacy, and help us better understand the efficacy required of CLC products. To explore this gap in knowledge, we used ATCC 30461 and ATCC 50370 trophozoites to examine behavior during and after CLC disinfection. Amoebae were added to sterile aluminum flow cells and flow cell solutions were changed to Ringer's solution (control), or one of four CLCs based on biocides (PHMB, PAPB/Polyquad, Polyquad/Aldox, or Polyquad/Alexidine) for 6 h. Each flow cell solution was then changed to axenic culture media (AC6) for 12 h to determine the behavior of amoebae following disinfection. Distance, speed, and displacement were calculated for each organism. As compared to the control of one-quarter Ringer's solution, each CLC significantly impacted motility in both the CLC and AC6 conditions. However, the amoebae challenged with the PHMB CLC traveled a significantly greater total distance than with the other three CLCs, indicating differences in effectiveness between biocides. Furthermore, amoebae regaining motility post-disinfection by CLCs were observed to travel considerable distances and thus could be considered dangerous to ocular health. We determined that while all CLCs produced a substantial or complete cessation of movement vs. the control condition during disinfection, those which relied on the Polyquad biocides were the most effective, and that any amoebae which survived disinfection were able to recover motility. Future examinations of these findings should include direct correlations between motility and viability, and how infectivity and motility may be related.
角膜炎是一种威胁视力的角膜感染，治疗极具挑战性。了解这种微生物在接触镜接触和消毒过程中的反应，有助于我们更好地理解其如何在接触镜盒中定殖，更深入地了解隐形眼镜护理液（CLC）的功效，并帮助我们更好地理解CLC产品所需的功效。为了探索这一知识空白，我们使用ATCC 30461和ATCC 50370滋养体来研究CLC消毒期间及之后的行为。将变形虫添加到无菌铝制流动池中，流动池溶液更换为林格氏液（对照），或更换为基于杀菌剂的四种CLC之一（聚六亚甲基双胍、聚氨基丙基双胍/Polyquad、Polyquad/醛肟或Polyquad/氯己定），持续6小时。然后将每个流动池溶液更换为无菌培养基（AC6），持续12小时，以确定消毒后变形虫的行为。计算每个生物体的移动距离、速度和位移。与四分之一林格氏液对照相比，每种CLC在CLC和AC6条件下均显著影响了运动能力。然而，用聚六亚甲基双胍CLC处理的变形虫移动的总距离明显大于其他三种CLC处理的变形虫，这表明杀菌剂之间的有效性存在差异。此外，观察到经CLC消毒后恢复运动能力的变形虫会移动相当长的距离，因此可能对眼部健康构成危险。我们确定，虽然所有CLC在消毒过程中与对照条件相比都使运动大幅减少或完全停止，但依赖Polyquad杀菌剂的CLC最有效，并且任何在消毒后存活的变形虫都能够恢复运动能力。对这些发现的未来研究应包括运动能力与生存能力之间的直接关联，以及感染性与运动能力可能如何相关。
