Campolo Allison, Shannon Paul, Crary Monica
Alcon Research, LLC, Fort Worth, TX 76134, USA.
Pathogens. 2021 Jan 27;10(2):126. doi: 10.3390/pathogens10020126.
keratitis (AK) is a serious ocular infection caused by a ubiquitous free-living amoeba, . This infection often results in extensive corneal damage and blindness, and is notoriously difficult to cure. While is an abundant organism, AK is most associated with contact lens hygiene noncompliance and inadequate contact lens care (CLC) disinfection regimens. Thus, accurate and timely antimicrobial efficacy testing of CLC solutions is paramount. Published methods for antimicrobial efficacy testing of trophozoites requires 14 days for results. Presently, alternate and/or rapid methods for evaluating CLC products rarely demonstrate equivalent results compared to commonly-reported methods. Propidium iodide is a cellular stain that can only bind to cells with damaged outer membranes. We evaluated propidium iodide staining as an alternative method for determining the relative antimicrobial efficacy of 11 different CLC products against trophozoites. Following exposure to a CLC product, the fluorescence intensity of propidium iodide in an population demonstrated a strong correlation to the log reduction determined by established, growth-based testing used to evaluate the antimicrobial efficacy of CLC products. Thus, propidium iodide was found to be an effective rapid tool for determining cell death in trophozoites following exposure to CLC solutions.
棘阿米巴角膜炎（AK）是一种由无处不在的自由生活变形虫引起的严重眼部感染。这种感染常常导致广泛的角膜损伤和失明，而且 notoriously 难以治愈。虽然 是一种常见的生物体，但AK大多与隐形眼镜卫生不达标以及隐形眼镜护理（CLC）消毒方案不当有关。因此，对CLC溶液进行准确及时的抗菌功效测试至关重要。已发表的针对滋养体的抗菌功效测试方法需要14天才能得出结果。目前，与常用方法相比，评估CLC产品的替代和/或快速方法很少能显示出等效结果。碘化丙啶是一种细胞染色剂，只能与外膜受损的细胞结合。我们评估了碘化丙啶染色作为一种替代方法，用于确定11种不同CLC产品对滋养体的相对抗菌功效。在接触CLC产品后，群体中碘化丙啶的荧光强度与通过用于评估CLC产品抗菌功效的既定基于生长的测试所确定的对数减少呈强相关。因此，发现碘化丙啶是一种有效的快速工具，用于确定接触CLC溶液后滋养体中的细胞死亡情况。
