Boanini Elisa, Panseri Silvia, Arroyo Fabiola, Montesi Monica, Rubini Katia, Tampieri Anna, Covarrubias Cristian, Bigi Adriana
Department of Chemistry "G. Ciamician", University of Bologna, Via Selmi 2, Bologna 40126, Italy.
Institute of Science and Technology for Ceramics, National Research Council of Italy, Via Granarolo 64, Faenza 48018, Italy.
Materials (Basel). 2016 Feb 26;9(3):135. doi: 10.3390/ma9030135.
In this work we synthesized mesoporous bioactive glass nanospheres (nMBG) with the aim to utilize them as substrates for loading one of the most potent amino-bisphosphonates, alendronate (AL). The results of the chemical and structural characterization show that the nMBG display a relatively high surface area (528 m²/g) and a mean pore volume of 0.63 cm³/g, both of which decrease on increasing alendronate content. It is possible to modulate the amount of AL loaded into the nanospheres up to a maximum value of about 17 wt %. tests were performed using a human osteosarcoma cell line (MG63) and a murine monocyte/macrophage cell line as osteoclast model (RAW 264.7). The results indicate that even the lower concentration of alendronate provokes decreased tumor cell viability, and that osteoclast activity exhibits an alendronate dose-dependent inhibition. The data suggest that nMBG can act as a suitable support for the local delivery of alendronate, and that the antiresorptive and antitumor properties of the functionalized mesoporous nanospheres can be modulated by varying the amount of alendronate loading.
在本研究中，我们合成了介孔生物活性玻璃纳米球（nMBG），旨在将其用作负载最有效的氨基双膦酸盐之一阿仑膦酸盐（AL）的载体。化学和结构表征结果表明，nMBG具有相对较高的比表面积（528 m²/g）和平均孔体积0.63 cm³/g，随着阿仑膦酸盐含量的增加，这两者均会降低。可以将负载到纳米球中的AL量调节至最大值约17 wt%。使用人骨肉瘤细胞系（MG63）和作为破骨细胞模型的小鼠单核细胞/巨噬细胞系（RAW 264.7）进行了测试。结果表明，即使是较低浓度的阿仑膦酸盐也会导致肿瘤细胞活力下降，并且破骨细胞活性呈现出阿仑膦酸盐剂量依赖性抑制。数据表明，nMBG可以作为阿仑膦酸盐局部递送的合适载体，并且可以通过改变阿仑膦酸盐负载量来调节功能化介孔纳米球的抗吸收和抗肿瘤特性。
