Department of Nutrition and Food Hygiene, School of Public Health, Peking University, No. 38 Xueyuan Road, Beijing 100083, China.
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Peking University, No. 38 Xueyuan Road, Beijing 100083, China.
Nutrients. 2023 Feb 9;15(4):888. doi: 10.3390/nu15040888.
Currently, it must be acknowledged that little is known about the quantity and make-up of oligosaccharides (OS) found in breast-fed babies' feces as well as their metabolic fate. In the present work, UPLC-QE-HF-MS was successfully adopted to identify the profiles of human milk oligosaccharides (HMOs) in the breast milk of four mothers and fecal OS in the feces of their breast-fed infant. There were significant variations and differences in both number and composition between HMOs and fecal OS. The early-life gastrointestinal microbiota metabolism may be triggered into the advanced breakdown, synthesis, bioconversion, or redesign of HMOs. The fate of HMOs during passage through the gastrointestinal tract may be profoundly informed by the comparison of OS between breast milk and fecal OS profiles. The characterization of fecal OS could be applied as a valuable tool for monitoring the gastrointestinal fate of HMOs and reflecting infant development at different stages of lactation. Further research on the gastrointestinal bioconversion of HMOs profiles is required, including secretor type and the lactation time of milk, as well as baby feeding.
目前，必须承认，人们对于母乳喂养婴儿粪便中寡糖（OS）的数量和组成以及其代谢命运知之甚少。在本研究中，采用 UPLC-QE-HF-MS 成功鉴定了 4 位母亲母乳中的人乳寡糖（HMO）和其母乳喂养婴儿粪便中的粪便 OS。HMO 和粪便 OS 的数量和组成均存在显著差异和不同。生命早期胃肠道微生物群代谢可能引发 HMO 的高级分解、合成、生物转化或重新设计。通过比较母乳和粪便 OS 谱中的 OS，可以更深入地了解 HMO 穿过胃肠道时的命运。粪便 OS 的特征可作为监测 HMO 胃肠道命运和反映不同哺乳阶段婴儿发育的有价值工具。需要进一步研究 HMO 谱的胃肠道生物转化，包括分泌型和乳汁分泌时间以及婴儿喂养方式。
