CNC-Center for Neuroscience and Cell Biology, UC-Biotech, University of Coimbra, 3060-197 Cantanhede, Portugal.
Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Nutrients. 2023 Feb 12;15(4):924. doi: 10.3390/nu15040924.
Obesity, a rising concern in the Eastern world, encompasses several co-morbidities, namely non-alcoholic fatty liver disease (NAFLD). Potential natural-based interventions to decrease the burden of obesity complications are being investigated. Many of the edible parts of plants are not sold for consumption and end up as massive waste, losing nutritional potential. In fact, a sizeable amount of waste is generated within the different steps of the food supply chain, representing a massive loss of both plant material and natural resources. A good example is by-products (BBPs). The objective of this work was to investigate the effect of three different extracts from broccoli ( var ) by-products in an model of free fatty acid (FFA)-induced lipotoxicity using human hepatoma HepG2 cells. Broccoli leaf, stalk, and inflorescence extracts induced a dose-dependent decrease in the cell viability of HepG2 cells. However, the maximal non-lethal concentrations of leaves, stalks, and inflorescences (10 μg/mL) did not compromise mitochondrial function or neutral lipid accumulation in HepG2 cells. The extracts significantly decreased FFA-induced lipid accumulation in HepG2 cells either in a co-incubation or pre-incubation strategy. The broccoli extracts' capacity to prevent the FFA-induced decrease in catalase activity in HepG2 may explain the observed effects.
肥胖是一个在东方世界日益受到关注的问题，它包含多种合并症，例如非酒精性脂肪性肝病（NAFLD）。目前正在研究一些潜在的基于天然的干预措施，以减轻肥胖并发症的负担。许多植物的可食用部分没有被销售用于消费，最终成为大量的废物，失去了营养潜力。事实上，在食品供应链的不同环节都会产生大量的废物，这代表着植物材料和自然资源的巨大损失。一个很好的例子是副产物（BBPs）。本研究的目的是研究三种不同的西兰花（ var ）副产物提取物对游离脂肪酸（FFA）诱导的脂毒性的影响，采用人肝癌 HepG2 细胞作为模型。西兰花叶、茎和花序提取物诱导 HepG2 细胞的细胞活力呈剂量依赖性下降。然而，叶片、茎和花序的最大非致死浓度（10 μg/mL）并不影响 HepG2 细胞中的线粒体功能或中性脂质积累。这些提取物在 HepG2 细胞中以共孵育或预孵育的策略显著减少了 FFA 诱导的脂质积累。西兰花提取物在 HepG2 细胞中预防 FFA 诱导的过氧化氢酶活性下降的能力可能解释了观察到的效果。
