Park Seon Kyeong, Ha Jeong Su, Kim Jong Min, Kang Jin Yong, Lee Du Sang, Guo Tian Jiao, Lee Uk, Kim Dae-Ok, Heo Ho Jin
Division of Applied Life Science (BK21 plus), Institute of Agriculture & Life Science, Gyeongsang National University , Jinju 52828, Republic of Korea.
Department of Special Purpose Trees, Korea Forest Research Institute , Suwon 16631, Republic of Korea.
J Agric Food Chem. 2016 May 4;64(17):3353-61. doi: 10.1021/acs.jafc.6b00559. Epub 2016 Apr 22.
To examine the antiamnesic effects of broccoli (Brassica oleracea var. italica) leaves, we performed in vitro and in vivo tests on amyloid beta (Aβ)-induced neurotoxicity. The chloroform fraction from broccoli leaves (CBL) showed a remarkable neuronal cell-protective effect and an inhibition against acetylcholinesterase (AChE). The ameliorating effect of CBL on Aβ1-42-induced learning and memory impairment was evaluated by Y-maze, passive avoidance, and Morris water maze tests. The results indicated improving cognitive function in the CBL group. After the behavioral tests, antioxidant effects were detected by superoxide dismutase (SOD), oxidized glutathione (GSH)/total GSH, and malondialdehyde (MDA) assays, and inhibition against AChE was also presented in the brain. Finally, oxo-dihydroxy-octadecenoic acid (oxo-DHODE) and trihydroxy-octadecenoic acid (THODE) as main compounds were identified by quadrupole time-of-flight ultraperformance liquid chromatography (Q-TOF UPLC-MS) analysis. Therefore, our studies suggest that CBL could be used as a natural resource for ameliorating Aβ1-42-induced learning and memory impairment.
为了研究西兰花（Brassica oleracea var. italica）叶的抗遗忘作用，我们对淀粉样β蛋白（Aβ）诱导的神经毒性进行了体外和体内试验。西兰花叶的氯仿提取物（CBL）表现出显著的神经元细胞保护作用，并能抑制乙酰胆碱酯酶（AChE）。通过Y迷宫、被动回避和莫里斯水迷宫试验评估了CBL对Aβ1-42诱导的学习和记忆损伤的改善作用。结果表明CBL组的认知功能得到改善。行为测试后，通过超氧化物歧化酶（SOD）、氧化型谷胱甘肽（GSH）/总谷胱甘肽和丙二醛（MDA）检测发现了抗氧化作用，并且在脑中也呈现出对AChE的抑制作用。最后，通过四极杆飞行时间超高效液相色谱（Q-TOF UPLC-MS）分析鉴定出主要化合物氧代二羟基十八碳烯酸（oxo-DHODE）和三羟基十八碳烯酸（THODE）。因此，我们的研究表明CBL可作为改善Aβ1-42诱导的学习和记忆损伤的天然资源。
