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用于皮肤伤口治疗的丝胶蛋白/人胎盘来源的细胞外基质支架——制备、表征及分析

Sericin/Human Placenta-Derived Extracellular Matrix Scaffolds for Cutaneous Wound Treatment-Preparation, Characterization, and Analyses.

作者信息

Bhoopathy Jayavardhini, Dharmalingam Sankari, Sathyaraj Weslen Vedakumari, Rajendran Selvarajan, Rymbai Shibormi, Senthil Rethinam, Atchudan Raji

机构信息

Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India.

Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India.

出版信息

Pharmaceutics. 2023 Jan 20;15(2):362. doi: 10.3390/pharmaceutics15020362.

DOI:10.3390/pharmaceutics15020362
PMID:36839684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9962400/
Abstract

Human placenta is loaded with an enormous amount of endogenous growth factors, thereby making it a superior biomaterial for tissue regeneration. Sericin is a naturally occurring silk protein that is extensively used for biomedical applications. In the present work, sericin and human placenta-derived extracellular matrix were blended and fabricated in the form of scaffolds using the freeze-drying method for cutaneous wound treatment. The prepared sericin/placenta-derived extracellular matrix (SPEM) scaffolds were characterized to determine their morphology, functional groups, mechanical strength, and antibacterial activity. Scanning electron microscopic analysis of the scaffolds showed smooth surfaces with interconnected pores. MTT and scratch wound assays performed using HaCaT cells proved the non-toxic and wound-healing efficacy of SPEM scaffolds. CAM assay using fertilized chick embryos proved the angiogenic potency of the scaffolds. Animal experiments using Wistar albino rats proved that the open excision wounds treated with SPEM scaffolds significantly reduced wound size with collagen deposition. These results confirm that SPEM scaffolds can serve as a promising biomaterial for tissue regeneration.

摘要

人胎盘富含大量内源性生长因子，因此使其成为用于组织再生的优质生物材料。丝胶蛋白是一种天然存在的丝蛋白，广泛应用于生物医学领域。在本研究中，将丝胶蛋白与人胎盘来源的细胞外基质混合，并采用冷冻干燥法制成支架用于皮肤伤口治疗。对制备的丝胶蛋白/胎盘来源的细胞外基质（SPEM）支架进行表征，以确定其形态、官能团、机械强度和抗菌活性。支架的扫描电子显微镜分析显示表面光滑且有相互连通的孔隙。使用HaCaT细胞进行的MTT和划痕伤口试验证明了SPEM支架的无毒及伤口愈合功效。使用受精鸡胚进行的鸡胚绒毛尿囊膜试验证明了支架的血管生成能力。使用Wistar白化大鼠进行的动物实验证明，用SPEM支架治疗的开放性切除伤口可显著减小伤口大小并伴有胶原蛋白沉积。这些结果证实，SPEM支架可作为一种有前景的组织再生生物材料。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/b127efff3b3c/pharmaceutics-15-00362-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/35670e218adf/pharmaceutics-15-00362-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/a0a1c7f0ac09/pharmaceutics-15-00362-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/b127efff3b3c/pharmaceutics-15-00362-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/436480713c2c/pharmaceutics-15-00362-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/df85fbf5a1f4/pharmaceutics-15-00362-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/69c7c08a1422/pharmaceutics-15-00362-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/114557c70094/pharmaceutics-15-00362-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c0/9962400/9f02963605e4/pharmaceutics-15-00362-g013.jpg

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