Department of Chemistry, Hindustan Institute of Technology and Science, Padur, Chennai 603 103, India.
Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, Chennai 603 103, India.
Int J Biol Macromol. 2020 Aug 15;157:135-145. doi: 10.1016/j.ijbiomac.2020.04.156. Epub 2020 Apr 24.
Three-dimensional (3D) porous films based on chitosan/pectin/ZnO nanoparticles (NPs) were prepared for wound healing by the freeze-drying method. The chemical nature, composition and morphology of these films were revealed by FTIR, XRD, EDX, SEM and BET analysis. SEM micrographs showed a decrease in the pore size and porosity of chitosan/pectin/ZnO films when increasing the content of ZnO NPs. The developed films presented the swelling degree and water retention ability in the range of 189-465 and 230-390%, respectively. Moreover, they showed an improved compression strength and controlled degradation in the lysozyme-containing medium in comparison with control. MTT assay demonstrated the biocompatibility of chitosan/pectin/ZnO films against the primary human dermal fibroblast cells (HFCs). Among the developed chitosan/pectin/ZnO films, CPZnO-2 films presented the increased rate of cell proliferation and migration. Also, they exhibited antimicrobial activity against the gram-positive and gram-negative bacteria and fungi. These results suggested that chitosan/pectin/ZnO films could be safe, convenient and effective for wound healing.
基于壳聚糖/果胶/氧化锌纳米粒子（NPs）的三维（3D）多孔膜通过冷冻干燥法制备用于伤口愈合。通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、能谱分析（EDX）、扫描电子显微镜（SEM）和 BET 分析揭示了这些薄膜的化学性质、组成和形态。SEM 显微照片显示，当增加 ZnO NPs 的含量时，壳聚糖/果胶/ZnO 薄膜的孔径和孔隙率减小。所开发的薄膜的溶胀度和保水能力分别在 189-465 和 230-390%的范围内。此外，与对照相比，它们在含溶菌酶的介质中表现出改善的压缩强度和受控降解。MTT 测定表明壳聚糖/果胶/ZnO 薄膜对原代人皮肤成纤维细胞（HFCs）具有生物相容性。在所开发的壳聚糖/果胶/ZnO 薄膜中，CPZnO-2 薄膜表现出增加的细胞增殖和迁移率。此外，它们对革兰氏阳性和革兰氏阴性细菌和真菌表现出抗菌活性。这些结果表明壳聚糖/果胶/ZnO 薄膜可安全、方便、有效地用于伤口愈合。
