Siddiqui Arif Jamal, Patel Mitesh, Adnan Mohd, Jahan Sadaf, Saxena Juhi, Alshahrani Mohammed Merae, Abdelgadir Abdelmushin, Bardakci Fevzi, Sachidanandan Manojkumar, Badraoui Riadh, Snoussi Mejdi, Ouhtit Allal
Department of Biology, College of Science, University of Ha'il, Ha'il P.O. Box 2440, Saudi Arabia.
Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, India.
Pharmaceutics. 2023 Jan 25;15(2):403. doi: 10.3390/pharmaceutics15020403.
The proteinaceous compounds produced by lactic acid bacteria are called bacteriocins and have a wide variety of bioactive properties. However, bacteriocin's commercial availability is limited due to short stability periods and low yields. Therefore, the objective of this study was to synthesize bacteriocin-derived silver nanoparticles (Bac10307-AgNPs) extracted from (), which may have the potential to increase the bioactivity of bacteriocins and overcome the hurdles. It was found that extracted and purified Bac10307 had a broad range of stability for both temperature (20-100 °C) and pH (3-12). Further, based on Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, its molecular weight was estimated to be 4.2 kDa. The synthesized Bac10307-AgNPs showed a peak of surface plasmon resonance at 430 nm λmax. Fourier transform infrared (FTIR) confirmed the presence of biological moieties, and transmission electron microscopy (TEM) coupled with Energy dispersive X-Ray (EDX) confirmed that AgNPs were spherical and irregularly shaped, with a size range of 9-20 nm. As a result, the Bac10307-AgNPs displayed very strong antibacterial activity with MIC values as low as 8 μg/mL for () and (), when compared to Bac10307 alone. In addition, Bac10307-AgNPs demonstrated promising in vitro antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC = 116.04 μg/mL) and in vitro cytotoxicity against human liver cancer cells (HepG2) (IC = 135.63 μg/mL), more than Bac10307 alone (IC = 139.82 μg/mL against DPPH and 158.20 μg/mL against HepG2). Furthermore, a protein-protein molecular docking simulation study of bacteriocins with target proteins of different biological functions was also carried out in order to ascertain the interactions between bacteriocins and target proteins.
乳酸菌产生的蛋白质类化合物被称为细菌素，具有多种生物活性特性。然而，由于稳定性期短和产量低，细菌素的商业可用性有限。因此，本研究的目的是合成从（）中提取的细菌素衍生银纳米颗粒（Bac10307-AgNPs），其可能具有提高细菌素生物活性并克服障碍的潜力。研究发现，提取并纯化的Bac10307在温度（20-100°C）和pH（3-12）范围内均具有广泛的稳定性。此外，基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，其分子量估计为4.2 kDa。合成的Bac10307-AgNPs在430 nm λmax处显示出表面等离子体共振峰。傅里叶变换红外光谱（FTIR）证实了生物部分的存在，透射电子显微镜（TEM）结合能量色散X射线（EDX）证实AgNPs为球形且形状不规则，尺寸范围为9-20 nm。结果，与单独的Bac10307相比，Bac10307-AgNPs对（）和（）显示出非常强的抗菌活性，MIC值低至8 μg/mL。此外，Bac10307-AgNPs对2,2-二苯基-1-苦基肼（DPPH）表现出有前景的数据体外抗氧化活性（IC = 116.04 μg/mL），对人肝癌细胞（HepG2）表现出数据体外细胞毒性（IC = 135.63 μg/mL），比单独的Bac10307更强（对DPPH为IC = 139.82 μg/mL，对HepG2为158.20 μg/mL）。此外，还进行了细菌素与不同生物学功能靶蛋白的蛋白质-蛋白质分子对接模拟研究，以确定细菌素与靶蛋白之间的相互作用。
