Abdelgadir Abdelmushin, Adnan Mohd, Patel Mitesh, Saxena Juhi, Alam Mohammad Jahoor, Alshahrani Mohammed Merae, Singh Ritu, Sachidanandan Manojkumar, Badraoui Riadh, Siddiqui Arif Jamal
Department of Biology, College of Science, University of Ha'il, Ha'il, P.O. Box 2440, Saudi Arabia.
Research and Development Cell, Department of Biotechnology, Parul Institute of Applied Sciences, Parul University, Vadodara, 391760, India.
Heliyon. 2024 Sep 17;10(18):e37987. doi: 10.1016/j.heliyon.2024.e37987. eCollection 2024 Sep 30.
Biogenic synthesis of silver nanoparticles (AgNPs) has emerged as an eco-friendly and sustainable approach with diverse biological applications. This study presents synthesis of AgNPs-LS using a probiotic strain () and explores their multifaceted biological activities, including antibacterial, antibiofilm, anti-quorum sensing, antifungal, antioxidant, anticancer, anticoagulant and thrombolytic properties. The biosynthesis of AgNPs-LS was successfully achieved using cell free supernatants, resulting in well-characterized nanoparticles as confirmed by UV-Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS) and zeta potential analysis. The AgNPs-LS demonstrated potent antibacterial activity against different pathogenic bacteria (, and ), emphasizing their potential in combating bacterial infections. Moreover, these AgNPs-LS were effective in inhibiting biofilm formation (>60 % at 1/2 MIC), a key mechanism of bacterial virulence, highlighting their utility in preventing biofilm-related infections. AgNPs-LS exhibited anti-quorum sensing activity, disrupting bacterial communication systems and potentially reducing virulence factor such as, violacein production in pyocyanin production in and prodigiosin production in . Additionally, AgNPs-LS also exhibited notable antifungal activity towards a different pathogenic fungus (). In terms of health applications, the AgNPs-LS displayed significant antioxidant activity, effectively scavenging DPPH (IC = 42.65 μg/mL) and ABTS (IC = 53.77 μg/mL) free radicals. Furthermore, AgNPs-LS showed cytotoxicity against breast cancer cells (MCF-7) (IC = 52.29 μg/mL), positioning them as promising candidates for cancer therapy. Moreover, AgNPs-LS were also shown promising anticoagulant and thrombolytic activities under practical conditions. Therefore, the biogenic synthesis of AgNPs-LS using offers a sustainable and cost-effective route for producing AgNPs with an array of biological activities. These AgNPs-LS have the potential to address various challenges in healthcare, ranging from antimicrobial, anticancer applications to biofilm inhibition, antioxidant therapy, anticoagulant and thrombolytic agents.
银纳米颗粒（AgNPs）的生物合成已成为一种具有多种生物应用的环保且可持续的方法。本研究介绍了使用益生菌菌株（）合成AgNPs-LS，并探索了它们多方面的生物活性，包括抗菌、抗生物膜、群体感应抑制、抗真菌、抗氧化、抗癌、抗凝和溶栓特性。使用无细胞上清液成功实现了AgNPs-LS的生物合成，通过紫外可见光谱、傅里叶变换红外（FTIR）光谱、透射电子显微镜（TEM）、动态光散射（DLS）和zeta电位分析证实，得到了特征明确的纳米颗粒。AgNPs-LS对不同的病原菌（、和）表现出强大的抗菌活性，强调了它们在对抗细菌感染方面的潜力。此外，这些AgNPs-LS在抑制生物膜形成方面有效（在1/2 MIC时>60%），这是细菌毒力的关键机制，突出了它们在预防生物膜相关感染方面的效用。AgNPs-LS表现出群体感应抑制活性，破坏细菌通信系统，并可能降低毒力因子，如在中紫菌素的产生、在中绿脓菌素的产生以及在中灵菌红素的产生。此外，AgNPs-LS对不同的致病真菌（）也表现出显著的抗真菌活性。在健康应用方面，AgNPs-LS表现出显著的抗氧化活性，有效清除DPPH（IC = 42.65 μg/mL）和ABTS（IC = 53.77 μg/mL）自由基。此外，AgNPs-LS对乳腺癌细胞（MCF-7）表现出细胞毒性（IC = 52.29 μg/mL），使其成为癌症治疗的有希望的候选者。此外，在实际条件下，AgNPs-LS还表现出有希望的抗凝和溶栓活性。因此，使用合成AgNPs-LS为生产具有一系列生物活性的AgNPs提供了一条可持续且经济高效的途径。这些AgNPs-LS有潜力应对医疗保健中的各种挑战，从抗菌、抗癌应用到生物膜抑制、抗氧化治疗、抗凝和溶栓剂。
