Vio Santiago A, Galar María Lina, Gortari María Cecilia, Balatti Pedro, Garbi Mariana, Lodeiro Aníbal Roberto, Luna María Flavia
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (CONICET/UNLP), Calle 50 227, La Plata 1900, Argentina.
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), Calle 526 e/ Calles 10 y 11, La Plata 1900, Argentina.
Plants (Basel). 2023 Feb 7;12(4):736. doi: 10.3390/plants12040736.
The use of multispecies bacterial bio-inputs is a promising strategy for sustainable crop production over the use of single-species inoculants. Studies of the use of multispecies bio-inputs in horticultural crops are scarce, not only on the growth-promoting effects of each bacterium within the formulation, but also on their compatibility and persistence in the root environment. In this work, we described that a multispecies bacterial bio-input made up of Az39, PAL-5, Pf-5 and sp. Dm-B10 improved lettuce plant growth more effectively than when these strains were inoculated as single-species bio-inputs. Bacteria persisted together (were compatible) and also colonized seedling roots of lettuce plants grown in controlled conditions. Interestingly, colonization was highly related to an early and enhanced growth of seedlings grown in the nursery. A similar effect on plant growth was found in lettuce plants in a commercial greenhouse production in the peri-urban area of La Plata City, Buenos Aires, Argentina. To our knowledge, this is the first study demonstrating that a synthetic mixture of bacteria can colonize and persist on lettuce plants, and also showing their synergistic beneficial effect both in the nursery greenhouse as well as the commercial production farm.
与使用单一物种接种剂相比，使用多物种细菌生物投入物是实现作物可持续生产的一种有前景的策略。关于在园艺作物中使用多物种生物投入物的研究很少，不仅涉及制剂中每种细菌的促生长作用，还包括它们在根际环境中的兼容性和持久性。在这项研究中，我们发现由Az39、PAL - 5、Pf - 5和Dm - B10菌株组成的多物种细菌生物投入物比将这些菌株作为单一物种生物投入物接种时更有效地促进了生菜植株的生长。这些细菌能够共同存活（具有兼容性），并且还能定殖于在可控条件下生长的生菜幼苗根系。有趣的是，定殖与苗圃中幼苗的早期生长增强高度相关。在阿根廷布宜诺斯艾利斯省拉普拉塔市城郊的商业温室生产中的生菜植株上也发现了类似的对植物生长的影响。据我们所知，这是第一项证明细菌的合成混合物能够在生菜植株上定殖并持续存在的研究，同时也展示了它们在苗圃温室和商业生产农场中的协同有益作用。
