Staszek Pawel, Piekarniak Maciej, Wal Agnieszka, Krasuska Urszula, Gniazdowska Agnieszka
Department of Plant Physiology, Institute of Biology, Warsaw University of Life Sciences-SGGW, 02-776 Warsaw, Poland.
Plants (Basel). 2023 Feb 8;12(4):755. doi: 10.3390/plants12040755.
The digestive fluid of pitcher plants is a rich source of enzymes and secondary metabolites, but its impact on higher plant growth and development remains unknown. The aim of the study was to determine the phytotoxicity of the digestive fluid of the pitcher plant () on the germination of tomato ( L.) seeds, elongation growth and cell viability of roots of tomato seedlings. The digestive fluid was collected from pitchers before feeding and four days after feeding; the pH and electrical conductivity of the fluid were determined. Undiluted and 50% fluids were used in the study. An inhibition of germination of tomato seeds, by around 30% and 55%, was respectively observed in 50% and 100% digestive fluids collected before and after feeding. Digestive fluid did not affect the root growth of tomato seedlings; a slight (6%) inhibition was only observed after the application of 100% digestive fluid from an unfed trap. The roots of the tomato seedlings treated with undiluted fluid were characterized by reduced cell viability. Reactive oxygen species (HO and O) were mainly localized in the root apex regardless of the used phytotoxic cocktail, and did not differ in comparison to control plants.
猪笼草的消化液富含酶和次生代谢产物，但其对高等植物生长发育的影响尚不清楚。本研究的目的是确定猪笼草消化液对番茄种子萌发、番茄幼苗根伸长生长和细胞活力的植物毒性。在喂食前和喂食后四天从捕虫笼中收集消化液；测定了消化液的pH值和电导率。研究中使用了未稀释和50%稀释的消化液。在喂食前和喂食后收集的50%和100%消化液中，分别观察到番茄种子萌发受到约30%和55%的抑制。消化液不影响番茄幼苗的根生长；仅在使用未喂食捕虫笼中的100%消化液后观察到轻微（6%）的抑制。用未稀释消化液处理的番茄幼苗根系细胞活力降低。无论使用何种植物毒性混合液，活性氧（HO和O）主要定位于根尖，与对照植物相比无差异。
