Shekari Farid, Shekari Fariborz, Najafi Javad, Abassi Amin, Radmanesh Zahra, Bones Atle Magnar
Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan 45371-38791, Iran.
Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh 83111-55181, Iran.
Plants (Basel). 2022 Oct 6;11(19):2629. doi: 10.3390/plants11192629.
Dodder ( Yunck.) is one of the most devastating parasitic plants, which reduces quantity and quality of crops. The inhibitory effect of catnip ( Benth.) extracts on germination and some seedling characteristics of the were investigated in three phases in a laboratory and greenhouse. Aqueous extracts from different organs of were used in bioassays. The extracts reduced germination percent, root and shoot growth, and dry weight of seedlings. Moreover, results showed an inhibitory effect of the extracts on the activity of alpha-amylase, protease, and beta-1,3-glucanase enzymes in germinating seeds. Under greenhouse conditions, seeds were planted with 30-day-old alfalfa plants and irrigated with extracts. The application of extracts from different organs of reduced emergence percent and length of stem and hampered attachment to alfalfa. extracts also inhibited the activity of antioxidant enzymes and increased the accumulation of hydrogen peroxide and the malondialdehyde in seedlings. The strongest inhibitory effects were observed from flower, leaf, and stem extracts of , respectively. However, after attachment to alfalfa plants, treatment by extracts did not exhibit any effect on infestation efficiency and growth traits. According to these findings, extract, especially from flower and leaf, may be recommended as a potent bio-control agent to control germination and early stage development of .
菟丝子（Yunck.）是最具破坏性的寄生植物之一，会降低作物的产量和质量。在实验室和温室分三个阶段研究了荆芥（Benth.）提取物对菟丝子发芽及一些幼苗特性的抑制作用。使用了荆芥不同器官的水提取物进行生物测定。荆芥提取物降低了菟丝子种子的发芽率、根和芽的生长以及幼苗的干重。此外，结果表明荆芥提取物对菟丝子发芽种子中的α-淀粉酶、蛋白酶和β-1,3-葡聚糖酶的活性有抑制作用。在温室条件下，将菟丝子种子与30日龄的苜蓿植株一起种植，并用荆芥提取物灌溉。施用荆芥不同器官的提取物降低了菟丝子的出苗率和茎长，并阻碍了菟丝子附着在苜蓿上。荆芥提取物还抑制了抗氧化酶的活性，并增加了菟丝子幼苗中过氧化氢和丙二醛的积累。分别从荆芥的花、叶和茎提取物中观察到最强的抑制作用。然而，在菟丝子附着到苜蓿植株上后，用荆芥提取物处理对侵染效率和菟丝子生长特性没有任何影响。根据这些发现，荆芥提取物，尤其是花和叶提取物，可推荐作为一种有效的生物防治剂来控制菟丝子的发芽和早期发育。
