Bertoldo Giovanni, Chiodi Claudia, Della Lucia Maria Cristina, Borella Matteo, Ravi Samathmika, Baglieri Andrea, Lucenti Piergiorgio, Ganasula Bhargava Krishna, Mulagala Chandana, Squartini Andrea, Concheri Giuseppe, Magro Francesco, Campagna Giovanni, Stevanato Piergiorgio, Nardi Serenella
Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), Campus of Agripolis, University of Padova, Viale dell'Università 16, 35020 Legnaro, Italy.
Crop Production and Biostimulation Laboratory, Interfacultary School of Bioengineers, Université Libre de Bruxelles, Campus Plaine CP 245, Bd du Triomphe, 1050 Brussels, Belgium.
Plants (Basel). 2023 Feb 13;12(4):843. doi: 10.3390/plants12040843.
The molecular and phenotypic effects of a brown seaweed extract (BSE) were assessed in sugar beet ( L.). Transcript levels of BSE-treated and untreated plants were studied by RNA-seq and validated by quantitative real-time PCR analysis (RT-qPCR). Root morphology, sugar yield, and processing quality traits were also analyzed to better elucidate the treatment effects. RNA-seq revealed 1019 differentially expressed genes (DEGs) between the BSE-treated and untreated plants. An adjusted -value < 0.1 and an absolute value of log2 (fold change) greater than one was used as criteria to select the DEGs. Gene ontology (GO) identified hormone pathways as an enriched biological process. Six DEGs involved in auxin and ABA pathways were validated using RT-qPCR. The phenotypic characterization indicated that BSE treatment led to a significant increase ( < 0.05) in total root length and the length of fine roots of plants grown under hydroponics conditions. The sugar yield of plants grown under field conditions was higher ( < 0.05) in the treated field plots compared with the control treatment, without impacting the processing quality. Our study unveiled the relevant effects of BSE application in regulating auxin- and ABA-related gene expression and critical traits related to sugar beet development and yield.
对一种褐藻提取物（BSE）在甜菜（Beta vulgaris L.）中的分子和表型效应进行了评估。通过RNA测序研究了经BSE处理和未处理植株的转录水平，并通过定量实时PCR分析（RT-qPCR）进行了验证。还分析了根形态、糖分产量和加工品质性状，以更好地阐明处理效果。RNA测序揭示了经BSE处理和未处理植株之间有1019个差异表达基因（DEG）。以调整后的P值<0.1和log2（倍数变化）的绝对值大于1作为选择DEG的标准。基因本体论（GO）将激素途径确定为一个富集的生物学过程。使用RT-qPCR验证了6个参与生长素和脱落酸途径的DEG。表型特征表明，BSE处理导致水培条件下生长的植株总根长和细根长度显著增加（P<0.05）。与对照处理相比，田间条件下生长的植株在处理后的田间小区中糖分产量更高（P<0.05），且不影响加工品质。我们的研究揭示了BSE应用在调节生长素和脱落酸相关基因表达以及与甜菜发育和产量相关的关键性状方面的相关效应。
