Gogacz Marek, Peszke Jerzy, Natorska-Chomicka Dorota, Ruszała Monika, Dos Santos Szewczyk Katarzyna
Chair and Department of Gynecology, Medical University of Lublin, 20-090 Lublin, Poland.
Department of Experimental Biotechnology, Decont LLC, 08-500 Ryki, Poland.
Plants (Basel). 2023 Feb 16;12(4):884. doi: 10.3390/plants12040884.
Propolis and its extracts show a wide spectrum of biological activity. Due to the necessity to use high temperatures and high polarity in the eluent, the obtained extracts are depleted of active compounds. The new, cold separation method allows obtaining a qualitatively better product containing a number of chemical compounds absent in extracts obtained using high-temperature methods. The purpose of our study was to evaluate the biological activity of propolis extracts produced with the cold separation method in four female breast cancer cell lines: MDA-MB-231, MDA-MB-468, MCF-7, and T-47D. The results of the breast cancer cell viability were obtained using the MTT test. Propolis extracts at 75 and 80% showed similar cytotoxicity against cancer cells, with the polyphenol fraction 75% being slightly more negative for cells. Propolis extracts at concentrations of 50, 75, and 100 µg/mL significantly reduced cell viability. With the exception of the MDA-MB-231 line, cell viability was also decreased after incubation with a concentration of 25 µg/mL. Our results suggest that propolis extracts obtained with the cold separation method may be considered as promising compounds for the production of health-promoting supplements.
蜂胶及其提取物具有广泛的生物活性。由于在洗脱过程中需要使用高温和高极性，所获得的提取物中活性化合物会减少。新的冷分离方法能够得到质量更好的产品，其中含有许多高温方法所得提取物中不存在的化合物。我们研究的目的是评估用冷分离方法制备的蜂胶提取物对四种女性乳腺癌细胞系（MDA-MB-231、MDA-MB-468、MCF-7和T-47D）的生物活性。使用MTT试验获得乳腺癌细胞活力的结果。75%和80%的蜂胶提取物对癌细胞表现出相似的细胞毒性，其中75%的多酚组分对细胞的负面影响略大。浓度为50、75和100μg/mL的蜂胶提取物显著降低细胞活力。除MDA-MB-231细胞系外，用25μg/mL浓度孵育后细胞活力也有所下降。我们的结果表明，用冷分离方法获得的蜂胶提取物可被视为生产促进健康补充剂的有前景的化合物。
