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从美国国家航空航天局(NASA)的六项任务中分离出的与航天器相关的微生物的基因组序列草图

Draft Genome Sequences of Spacecraft-Associated Microbes Isolated from Six NASA Missions.

作者信息

Tran Michelle T, Seuylemezian Arman, Wright Alonna, Coil David, Eisen Jonathan, Guan Lisa

机构信息

California State Polytechnic University, Pomona, California, USA.

Biotechnology and Planetary Protection, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, USA.

出版信息

Microbiol Resour Announc. 2023 Mar 16;12(3):e0101122. doi: 10.1128/mra.01011-22. Epub 2023 Feb 22.

DOI:10.1128/mra.01011-22
PMID:36840549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10019167/
Abstract

Whole-genome sequencing can be used to better understand and assess the functional abilities of microorganisms isolated from spacecraft hardware and associated surfaces for planetary protection (PP) purposes. We sequenced 191 isolates from 6 spaceflight missions with PP requirements and identified them using Illumina-based sequencing methods and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry.

摘要

全基因组测序可用于更好地理解和评估从航天器硬件及相关表面分离出的微生物的功能能力,以实现行星保护(PP)目的。我们对来自6次有行星保护要求的太空飞行任务的191株分离菌进行了测序,并使用基于Illumina的测序方法和基质辅助激光解吸电离飞行时间(MALDI-TOF)质谱对它们进行了鉴定。

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