Algamdi Abdullah G, Shaher Fekri M, Mahyoub Jazem A
Department of Biology Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Hodeidah University, Hodeidah, Republic of Yemen.
Saudi J Biol Sci. 2023 Mar;30(3):103581. doi: 10.1016/j.sjbs.2023.103581. Epub 2023 Feb 2.
In this study, samples of -infected mosquitoes were collected from Al-Safa district in Jeddah city, Saudi Arabia. The presence of bacteria in mosquitoes was confirmed by PCR technique and they were reared and propagated in the laboratory. Comparative studies were conducted between -infected and the -uninfected laboratory strain in terms of their ability to withstand drought, resist two types of insecticides and the activities of pesticide detoxification enzymes. The Wolbachia-infected strain proved less able to withstand the drought period, as the egg-hatching rate of the Wolbachia-uninfected strain was greater than that of the Wolbachia-infected strain after one, two and three months of dry periods. Compared to the uninfected strain, the -infected strain demonstrated a relatively greater resistance to tested pesticides, namely Baton 100EC and Fendure 25EC which may be attributed to the higher levels of the detoxification enzymes glutathione-S-transferase and catalase and the lower levels of esterase and acetylcholine esterase.
在本研究中，从沙特阿拉伯吉达市的Al-Safa区采集了感染[细菌名称未给出]的蚊子样本。通过PCR技术确认了蚊子体内存在[细菌名称未给出]细菌，并在实验室中进行饲养和繁殖。对感染[细菌名称未给出]的蚊子和未感染的实验室菌株在耐受干旱能力、抵抗两种杀虫剂能力以及农药解毒酶活性方面进行了比较研究。结果表明，感染沃尔巴克氏体的菌株耐受干旱期的能力较弱，因为在干旱期1个月、2个月和3个月后，未感染沃尔巴克氏体的菌株的卵孵化率高于感染沃尔巴克氏体的菌株。与未感染菌株相比，感染[细菌名称未给出]的菌株对测试农药（即Baton 100EC和Fendure 25EC）表现出相对更强的抗性，这可能归因于解毒酶谷胱甘肽-S-转移酶和过氧化氢酶的水平较高，而酯酶和乙酰胆碱酯酶的水平较低。
