van Haaren M J, Sedee N J, Schilperoort R A, Hooykaas P J
Department of Plant Molecular Biology, Leiden University, The Netherlands.
Nucleic Acids Res. 1987 Nov 11;15(21):8983-97. doi: 10.1093/nar/15.21.8983.
Introduction of a left or right synthetic border repeat together with the overdrive sequence in an octopine Ti-plasmid deletion mutant, lacking the right border, resulted in the complete restoration of the oncogenicity of the mutant strain. However introduction of a border repeat without the overdrive, only restored oncogenicity partially. The overdrive sequence turned out to be able to stimulate the synthetic border mediated T-region transfer, independent of its orientation and position relative to the border repeat. Furthermore the distance between border repeat and overdrive could be enlarged, without a loss of overdrive activity. Here we enlarged the distance between the two sequences up to 6714bp. These results were confirmed by estimating the amount of single stranded T-DNA molecules from induced agrobacteria, containing the various border constructs.
在一个缺乏右边界的章鱼碱Ti质粒缺失突变体中引入左或右合成边界重复序列以及超驱动序列，导致突变菌株的致癌性完全恢复。然而，引入没有超驱动的边界重复序列，仅部分恢复了致癌性。结果表明，超驱动序列能够刺激合成边界介导的T区转移，而与其相对于边界重复序列的方向和位置无关。此外，边界重复序列和超驱动之间的距离可以增大，而不会丧失超驱动活性。在这里，我们将两个序列之间的距离扩大到了6714bp。通过估计含有各种边界构建体的诱导农杆菌中单链T-DNA分子的数量，证实了这些结果。
