Shurvinton C E, Ream W
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-6502.
J Bacteriol. 1991 Sep;173(17):5558-63. doi: 10.1128/jb.173.17.5558-5563.1991.
T-DNA transfer by Agrobacterium tumefaciens depends on the right border repeat of the T-DNA and is greatly stimulated by overdrive, an adjacent sequence. We report that the function of overdrive does not depend on helical position with respect to the border repeat. A synthetic 24-bp overdrive and a 12-bp region containing a fully conserved 8-bp core overdrive sequence stimulated virulence equally, but full function required additional bases to the left of the 24-bp sequence.
根癌农杆菌介导的T-DNA转移依赖于T-DNA的右边界重复序列,并且受到其相邻序列——超驱动序列的强烈刺激。我们报道,超驱动序列的功能并不取决于其相对于边界重复序列的螺旋位置。一个合成的24碱基对超驱动序列和一个包含完全保守的8碱基对核心超驱动序列的12碱基对区域刺激毒性的效果相同,但完整功能需要24碱基对序列左侧的额外碱基。