Lebkowski J S, McNally M A, Okarma T B, Lerch L B
Applied ImmuneSciences, Inc., Menlo Park, CA 94025.
Nucleic Acids Res. 1987 Nov 11;15(21):9043-55. doi: 10.1093/nar/15.21.9043.
Phorbol ester tumor promoters affect a broad scope of changes in mammalian cells. This report describes the activation of expression of an introduced chloramphenicol acetyltransferase (CAT) reporter gene by the phorbol ester, phorbol 12-myristate 13-acetate (PMA), in a variety of fibroblast and hematopoietic cell lines. PMA-mediated activation appears to be promoter region specific, yet widespread. Enhanced gene expression is observed for four out of five promoter systems tested, and, in some cases, is dependent on the cellular environment. Further experiments indicate that PMA mediates elevated gene expression by rapidly increasing steady state levels of CAT mRNA. The broad range of promoters affected by PMA may help explain the high potency of this agent in tumor production.
佛波酯肿瘤启动子会影响哺乳动物细胞的广泛变化。本报告描述了佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）在多种成纤维细胞和造血细胞系中对导入的氯霉素乙酰转移酶（CAT）报告基因表达的激活作用。PMA介导的激活似乎具有启动子区域特异性，但范围广泛。在所测试的五个启动子系统中，有四个观察到基因表达增强，并且在某些情况下，这取决于细胞环境。进一步的实验表明，PMA通过迅速提高CAT mRNA的稳态水平来介导基因表达升高。受PMA影响的启动子范围广泛，这可能有助于解释该试剂在肿瘤发生中的高效性。
