Nealon Nora Jean, Wood Alexandra, Rudinsky Adam J, Klein Hannah, Salerno Matthew, Parker Valerie J, Quimby Jessica M, Howard James, Winston Jenessa A
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Front Vet Sci. 2023 Feb 8;10:1039931. doi: 10.3389/fvets.2023.1039931. eCollection 2023.
Fecal diagnostics are a mainstay of feline medicine, and fecal identification markers help to distinguish individuals in a multi-cat environment. However, the impact of identification markers on the fecal microbiota are unknown. Given the increased interest in using microbiota endpoints to inform diagnosis and treatment, the objective of this study was to examine the effects of orally supplemented glitter and crayon shavings on the feline fecal microbiota (amplicon sequencing of 16S rRNA gene V4 region). Fecal samples were collected daily from six adult cats that were randomized to receive oral supplementation with either glitter or crayon for two weeks, with a two-week washout before receiving the second marker. No adverse effects in response to marker supplementation were seen for any cat, and both markers were readily identifiable in the feces. Microbiota analysis revealed idiosyncratic responses to fecal markers, where changes in community structure in response to glitter or crayon could not be readily discerned. Given these findings, it is not recommended to administered glitter or crayon shavings as a fecal marker when microbiome endpoints are used, however their clinical use with other diagnostics should still be considered.
粪便诊断是猫科医学的主要手段，粪便识别标记有助于在多猫环境中区分个体。然而，识别标记对粪便微生物群的影响尚不清楚。鉴于人们越来越关注利用微生物群终点来指导诊断和治疗，本研究的目的是研究口服补充亮片和蜡笔屑对猫粪便微生物群的影响（16S rRNA基因V4区域的扩增子测序）。每天从六只成年猫收集粪便样本，这些猫被随机分为两组，分别口服补充亮片或蜡笔，持续两周，在接受第二种标记物之前有两周的洗脱期。没有观察到任何一只猫对标记物补充有不良反应，并且两种标记物在粪便中都很容易识别。微生物群分析显示对粪便标记物有特异反应，无法轻易辨别出因亮片或蜡笔导致的群落结构变化。鉴于这些发现，当使用微生物组终点时，不建议将亮片或蜡笔屑作为粪便标记物给药，然而它们与其他诊断方法的临床应用仍应予以考虑。
