Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Animal Virome and Diagnostic Development Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Vet Q. 2023 Dec;43(1):1-10. doi: 10.1080/01652176.2023.2185696.
Carnivore chaphamaparvovirus-1 (CaChPV-1) is a parvovirus identified in dogs and association of infection with diarrhea is controversial. Information on whether tissue tropism persists is lacking.
To determine the disease association of CaChPV-1 in dogs with diarrhea and to investigate viral tropism and genetic diversity.
CaChPV-1 infection was investigated in five recently deceased puppies and designed a retrospective study to determine whether the presence of CaChPV-1 is associated with diarrhea. The retrospective study was conducted in 137 intestinal tissue samples and 168 fecal samples obtained from 305 dogs. CaChPV-1 tissue localization was determined using hybridization, and CaChPV-1 complete genomes obtained from dead puppies and retrospective study were sequenced and analyzed.
CaChPV-1 was detected in 6.56% (20/305) of tested dogs, including 14 diarrheic- and 6 non-diarrheic dogs, and was significant in puppies with diarrhea ( = 0.048). Among the CaChPV-1-positive diarrheic dogs, one sample was obtained from intestinal tissue and 13 samples were fecal samples. However, six CaChPV-1 positive non-diarrheic dogs were based on fecal samples but not on intestinal tissue. Within the age range, the presence of CaChPV-1 was significant in puppies ( < 0.00001) and was mainly localized in the stromal and endothelial cells of intestinal villi and pulmonary alveoli. Phylogenetic analysis indicated genetic diversity of CaChPV-1 Thai strains that were mostly clustered within the sequences found in China.
Although definitive pathogenesis of CaChPV-1 remains undetermined, this study provides evidence supporting that CaChPV-1 localizes in canine cells and could play a potential role as an enteric pathogen.
Carnivore chaphamaparvovirus-1（CaChPV-1）是一种在狗中发现的细小病毒，其感染与腹泻的关联存在争议。关于其组织嗜性是否持续存在的信息尚不清楚。
确定感染 CaChPV-1 的犬腹泻的疾病关联，并研究病毒的嗜性和遗传多样性。
研究了五只最近死亡的幼犬中的 CaChPV-1 感染情况，并设计了一项回顾性研究以确定 CaChPV-1 的存在是否与腹泻有关。该回顾性研究在 305 只狗的 137 个肠组织样本和 168 个粪便样本中进行。使用杂交技术确定 CaChPV-1 的组织定位，并对从死亡幼犬和回顾性研究中获得的 CaChPV-1 完整基因组进行测序和分析。
在 305 只测试犬中，有 6.56%（20/305）检测到 CaChPV-1，包括 14 只腹泻犬和 6 只非腹泻犬，且腹泻犬中的检出率有统计学意义（ = 0.048）。在 CaChPV-1 阳性腹泻犬中，1 个样本来自肠组织，13 个样本来自粪便。然而，6 只 CaChPV-1 阳性非腹泻犬仅基于粪便样本，而不是肠组织样本。在年龄范围内，CaChPV-1 在幼犬中的存在有统计学意义（ < 0.00001），主要定位于肠绒毛和肺泡的间质和内皮细胞中。系统进化分析表明，泰国 CaChPV-1 株具有遗传多样性，主要聚类在在中国发现的序列内。
尽管 CaChPV-1 的明确发病机制尚不清楚，但本研究提供了支持 CaChPV-1 定殖于犬细胞并可能作为肠病原体发挥潜在作用的证据。
