环氧化酶-2 抑制剂联合（-）-表没食子儿茶素没食子酸酯或多酚 E 对 A/J 小鼠顺铂诱导肺肿瘤发生的影响。

Effect of a Cyclooxygenase-2 Inhibitor in Combination with (-)-Epigallocatechin Gallate or Polyphenon E on Cisplatin-Induced Lung Tumorigenesis in A/J Mice.

机构信息

Department of Hematology, Oncology, and Respiratory Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences.

Department of General Internal Medicine 4, Kawasaki Medical School.

出版信息

Acta Med Okayama. 2023 Feb;77(1):65-70. doi: 10.18926/AMO/64363.

DOI:10.18926/AMO/64363

PMID:36849147

Abstract

We investigated the effects of celecoxib combined with (-)-epigallocatechin-3-gallate (EGCG) or polyphenon E in a cisplatin-induced lung tumorigenesis model. Four-week-old female A/J mice were divided into seven groups: (i) Control, (ii) 150 mg/kg celecoxib (150Cel), (iii) 1,500 mg/kg celecoxib (1500Cel), (iv) EGCG+150 mg/kg celecoxib (EGCG+150Cel), (v) EGCG+1,500 mg/kg celecoxib (EGCG+1500Cel), (vi) polyphenon E+150 mg/kg celecoxib (PolyE+150Cel), and (vii) polyphenon E+1,500 mg/kg celecoxib (PolyE+1500Cel). All mice were administered cisplatin (1.62 mg/kg of body weight, i.p.) 1×/week for 10 weeks and sacrificed at week 30; the numbers of tumors on the lung surface were then determined. The tumor incidence and multiplicity (no. of tumors/mouse, mean±SD) were respectively 95% and 2.15±1.50 in Control, 95% and 2.10±1.29 in 150Cel, 86% and 1.67±1.20 in 1500Cel, 71% and 1.38±1.24 in EGCG+150Cel, 67% and 1.29±1.38 in EGCG+1500Cel, 80% and 1.95±1.36 in PolyE+150Cel, and 65% and 1.05±0.10 in PolyE+1500Cel. The combination of high-dose celecoxib with EGCG or polyphenon E significantly reduced multiplicity in cisplatin-induced lung tumors.

摘要

我们在顺铂诱导的肺癌发生模型中研究了塞来昔布联合（-）-表没食子儿茶素-3-没食子酸酯（EGCG）或多酚 E 的效果。将 4 周龄雌性 A/J 小鼠分为 7 组：（i）对照组，（ii）150mg/kg 塞来昔布（150Cel），（iii）1500mg/kg 塞来昔布（1500Cel），（iv）EGCG+150mg/kg 塞来昔布（EGCG+150Cel），（v）EGCG+1500mg/kg 塞来昔布（EGCG+1500Cel），（vi）多酚 E+150mg/kg 塞来昔布（PolyE+150Cel），和（vii）多酚 E+1500mg/kg 塞来昔布（PolyE+1500Cel）。所有小鼠每周接受 1 次顺铂（1.62mg/kg 体重，腹腔内注射）10 周，并在第 30 周处死；然后确定肺表面肿瘤的数量。对照组的肿瘤发生率和多发性（肿瘤数/只，平均值±标准差）分别为 95%和 2.15±1.50，150Cel 组为 95%和 2.10±1.29，1500Cel 组为 86%和 1.67±1.20，EGCG+150Cel 组为 71%和 1.38±1.24，EGCG+1500Cel 组为 67%和 1.29±1.38，PolyE+150Cel 组为 80%和 1.95±1.36，PolyE+1500Cel 组为 65%和 1.05±0.10。高剂量塞来昔布与 EGCG 或多酚 E 的联合使用显著降低了顺铂诱导的肺癌肿瘤的多发性。