Department of Hematology, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Cheeloo College of Medicine, Shandong University, Jinan, China.
Int J Lab Hematol. 2023 Aug;45(4):481-488. doi: 10.1111/ijlh.14041. Epub 2023 Feb 27.
Immune thrombocytopenia (ITP) is a common acquired hemorrhagic disease without "gold standard" for the diagnosis, long non-coding RNA (lncRNAs) can participate in regulating gene expression through various mechanisms and may play a role in immune intolerance in ITP. Several previous studies have confirmed that lncRNA lncDC and THRIL are involved in the development of autoimmune diseases. This study investigates the relationship between expression levels of two plasma lncRNAs (lncDC and THRIL) and clinical characteristics of adult primary ITP patients, ascertain their potential applications as diagnostic markers of ITP.
We recruited 102 subjects, including 41 ITP patients, 41 healthy controls (HCs) and 20 patients under myelosuppression phase after chemotherapy (MS). qRT-PCR was used to detect the expression of two lncRNAs in the peripheral blood plasma of the three groups. Receiver operating characteristic (ROC) curves were used to test the diagnostic efficacy of lncDC and THRIL in ITP.
The expression level of lncDC was downregulated in ITP patients compared with HCs (p = . 012) and MS (p = .035), whereas THRIL was significantly upregulated (p = .0005, p < . 0001). We further revealed that lncDC has a high sensitivity (78. 05%), while THRIL has a high specificity (97. 56%). The area under the curve (AUC) (0.869, 95% CI: 0.795-0.943, p < .0001) of the ROC curve for this combination increased significantly.
THRIL and lncDC expression levels were changed in adult ITP patients. The lncRNAs lncDC and THRIL can serve as potential diagnostic markers for adult primary ITP.
免疫性血小板减少症（ITP）是一种常见的获得性出血性疾病，目前尚无“金标准”用于诊断。长链非编码 RNA（lncRNAs）可以通过多种机制参与调节基因表达，并且可能在 ITP 的免疫耐受中发挥作用。一些先前的研究已经证实，lncRNA lncDC 和 THRIL 参与了自身免疫性疾病的发展。本研究旨在探讨两种血浆 lncRNA（lncDC 和 THRIL）的表达水平与成人原发性 ITP 患者临床特征之间的关系，确定其作为 ITP 诊断标志物的潜在应用价值。
我们招募了 102 名受试者，包括 41 名 ITP 患者、41 名健康对照者（HCs）和 20 名化疗后骨髓抑制期（MS）患者。qRT-PCR 用于检测三组患者外周血血浆中两种 lncRNA 的表达水平。采用受试者工作特征（ROC）曲线检测 lncDC 和 THRIL 在 ITP 中的诊断效能。
与 HCs（p=0.012）和 MS（p=0.035）相比，ITP 患者的 lncDC 表达水平下调，而 THRIL 表达水平显著上调（p=0.0005，p<0.0001）。我们进一步发现，lncDC 具有较高的灵敏度（78.05%），而 THRIL 具有较高的特异性（97.56%）。该联合 ROC 曲线的曲线下面积（AUC）（0.869，95%CI：0.795-0.943，p<0.0001）显著增加。
成人 ITP 患者中 lncDC 和 THRIL 的表达水平发生改变。lncRNAs lncDC 和 THRIL 可以作为成人原发性 ITP 的潜在诊断标志物。
