Bayat Mahnaz, Hooshmandi Etrat, Karimi Najmeh, Rahimi Moosa, Tabrizi Reza, Asadabadi Tahereh, Salehi Mohammad Saied, Zafarmand Seyedeh Shaghayegh, Owjfard Maryam, Garcia Esperon Carlos, Spratt Neil, Levi Christopher, Borhani-Haghighi Afshin
Clinical Neurology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran.
Curr J Neurol. 2024 Jan 5;23(1):74-82. doi: 10.18502/cjn.v23i1.16437.
Inflammation is the major contributor to the pathophysiology of ischemic stroke (IS). Long non-coding ribonucleic acids (lncRNAs) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and tumor necrosis factor and heterogeneous nuclear ribonucleoprotein L-related immunoregulatory (THRIL) have been demonstrated to be up-regulated in inflammation and atherosclerosis. Therefore, we aimed to study the expression profile of these lncRNAs after IS. This observational case-control study was conducted in Namazi Hospital, Shiraz, Iran. The real-time polymerase chain reaction (RT-PCR) measured the sequential changes in circulating levels of MALAT1 and THRIL on days 1, 3, and 5 after IS. The receiver operating characteristic (ROC) curve analysis was used to estimate the diagnostic and prognostic potential of lncRNAs with the area under the curve (AUC). In patients with IS, the relative MALAT1 and THRIL expressions were significantly higher than the controls (P < 0.001 and P < 0.01, respectively), on days 1, 3, and 5 after stroke. We showed a significantly increase in lncRNAs expression on day five compared to days 1 and 3 after stroke. Moreover, a positive correlation was detected between MALAT1 expression and time within the first 24 hours after stroke (r = 0.27, P = 0.03). Logistic regression analysis showed a significant positive association between MALAT1 and THRIL and the risk of stroke evolution. We found a potential diagnostic marker for MALAT1 with an AUC of 0.78. We demonstrated the significant sequential upregulation in MALAT1 and THRIL expression on days 1, 3, and 5 after IS with a significant positive association with the risk of stroke. MALAT1 also significantly correlated with time within the first 24 hours after stroke.
炎症是缺血性中风（IS）病理生理学的主要促成因素。长链非编码核糖核酸（lncRNAs）转移相关肺腺癌转录本1（MALAT1）以及肿瘤坏死因子和异质性核糖核蛋白L相关免疫调节因子（THRIL）已被证明在炎症和动脉粥样硬化中上调。因此，我们旨在研究这些lncRNAs在IS后的表达谱。这项观察性病例对照研究在伊朗设拉子的纳马齐医院进行。实时聚合酶链反应（RT-PCR）测定了IS后第1、3和5天循环中MALAT1和THRIL水平的连续变化。采用受试者工作特征（ROC）曲线分析，通过曲线下面积（AUC）评估lncRNAs的诊断和预后潜力。在IS患者中，中风后第1、3和5天，MALAT1和THRIL的相对表达显著高于对照组（分别为P < 0.001和P < 0.01）。我们发现，与中风后第1天和第3天相比，lncRNAs在第5天的表达显著增加。此外，还检测到MALAT1表达与中风后最初24小时内的时间呈正相关（r = 0.27，P = 0.03）。逻辑回归分析显示，MALAT1和THRIL与中风进展风险之间存在显著正相关。我们发现MALAT1有一个潜在的诊断标志物，AUC为0.78。我们证明了IS后第1、3和5天MALAT1和THRIL表达的显著连续上调，且与中风风险呈显著正相关。MALAT1也与中风后最初24小时内的时间显著相关。
