• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于牙周组织工程的微生物聚（3-羟基丁酸酯-共-3-羟基戊酸酯）支架

Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering.

作者信息

Phuegyod Seubsakul, Pramual Sasivimon, Wattanavichean Nungnit, Assawajaruwan Supasuda, Amornsakchai Taweechai, Sukho Panithi, Svasti Jisnuson, Surarit Rudee, Niamsiri Nuttawee

机构信息

Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand.

出版信息

Polymers (Basel). 2023 Feb 9;15(4):855. doi: 10.3390/polym15040855.

DOI:10.3390/polym15040855
PMID:36850140
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9962980/
Abstract

In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL). The water contact angle results indicated a hydrophobic character for all polymeric films. All fabricated scaffolds exhibited a high porosity of 90% with a sponge-like appearance. The P(HB-50HV) scaffolds were distinctively different in compressive modulus and was the material with the lowest stiffness among all scaffolds tested between the dry and wet conditions. The human gingival fibroblasts (HGFs) and periodontal ligament stem cells (PDLSCs) cultured onto the P(HB-50HV) scaffold adhered to the scaffold and exhibited the highest proliferation with a healthy morphology, demonstrating excellent cell compatibility with P(HB-50HV) scaffolds. These results indicate that the P(HB-50HV) scaffold could be applied as a biomaterial for periodontal tissue engineering and stem cell applications.

摘要

在本研究中，我们制备了具有50% HV含量的聚（羟基丁酸酯-共-羟基戊酸酯）三维（3D）多孔支架。P(HB-50HV)由细菌H16生物合成，并评估了用于组织工程应用的牙科细胞的体外增殖情况。将其与由聚（羟基丁酸酯）（PHB）、聚（羟基丁酸酯-共-12%羟基戊酸酯）（P(HB-12HV)）和聚己内酯（PCL）制备的支架进行了比较。水接触角结果表明所有聚合物薄膜均具有疏水性。所有制备的支架均呈现出90%的高孔隙率，外观呈海绵状。P(HB-50HV)支架在压缩模量方面有显著差异，是在干湿条件下测试的所有支架中刚度最低的材料。接种在P(HB-50HV)支架上培养的人牙龈成纤维细胞（HGFs）和牙周膜干细胞（PDLSCs）附着在支架上，并呈现出最高的增殖率，形态健康，表明与P(HB-50HV)支架具有良好的细胞相容性。这些结果表明，P(HB-50HV)支架可作为牙周组织工程和干细胞应用的生物材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/8265526d4b31/polymers-15-00855-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/b90be8709201/polymers-15-00855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/006240f925bb/polymers-15-00855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/b09eb69bcdbd/polymers-15-00855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/3055c58f70d0/polymers-15-00855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/cf7dad4cf212/polymers-15-00855-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/d3be03cedb0e/polymers-15-00855-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/8265526d4b31/polymers-15-00855-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/b90be8709201/polymers-15-00855-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/006240f925bb/polymers-15-00855-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/b09eb69bcdbd/polymers-15-00855-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/3055c58f70d0/polymers-15-00855-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/cf7dad4cf212/polymers-15-00855-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/d3be03cedb0e/polymers-15-00855-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1375/9962980/8265526d4b31/polymers-15-00855-g007.jpg

相似文献

1
Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering.用于牙周组织工程的微生物聚（3-羟基丁酸酯-共-3-羟基戊酸酯）支架
Polymers (Basel). 2023 Feb 9;15(4):855. doi: 10.3390/polym15040855.
2
Fabricated polyhydroxyalkanoates blend scaffolds enhance cell viability and cell proliferation.合成的聚羟基烷酸酯共混支架提高细胞活力和增殖。
J Biotechnol. 2023 Jan 10;361:30-40. doi: 10.1016/j.jbiotec.2022.11.014. Epub 2022 Nov 24.
3
Poly-3-hydroxybutyrate-co-3-hydroxyvalerate containing scaffolds and their integration with osteoblasts as a model for bone tissue engineering.含聚3-羟基丁酸酯-共-3-羟基戊酸酯的支架及其与成骨细胞的整合作为骨组织工程模型
J Biomater Appl. 2015 May;29(10):1394-406. doi: 10.1177/0885328214568467. Epub 2015 Jan 14.
4
Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate--3-hydroxyvalerate).基于聚（3-羟基丁酸酯）和聚（3-羟基丁酸酯-3-羟基戊酸酯）的多孔支架的制备与表征
Life (Basel). 2021 Sep 8;11(9):935. doi: 10.3390/life11090935.
5
Additive Manufacturing of Poly(3-hydroxybutyrate--3-hydroxyvalerate)/Poly(D,L-lactide--glycolide) Biphasic Scaffolds for Bone Tissue Regeneration.聚（3-羟基丁酸酯-3-羟基戊酸酯）/聚（D，L-丙交酯-乙交酯）双相支架的骨组织再生的增材制造。
Int J Mol Sci. 2022 Mar 31;23(7):3895. doi: 10.3390/ijms23073895.
6
Improved vascularisation but inefficient in vivo bone regeneration of adipose stem cells and poly-3-hydroxybutyrate-co-3-hydroxyvalerate scaffolds in xeno-free conditions.在无动物源条件下，脂肪干细胞和聚 3-羟基丁酸酯-3-羟基戊酸酯支架的血管化得到改善，但体内骨再生效率低下。
Mater Sci Eng C Mater Biol Appl. 2020 Feb;107:110301. doi: 10.1016/j.msec.2019.110301. Epub 2019 Oct 12.
7
Preparation of Porous Scaffold Based on Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) and FucoPol.基于聚（3-羟基丁酸酯-共-3-羟基戊酸酯-共-3-羟基己酸酯）和岩藻聚糖制备多孔支架
Polymers (Basel). 2023 Jul 4;15(13):2945. doi: 10.3390/polym15132945.
8
Design and fabrication of bone tissue scaffolds based on PCL/PHBV containing hydroxyapatite nanoparticles: dual-leaching technique.基于含纳米羟基磷灰石的聚己内酯/聚（丁二酸丁二醇酯）的骨组织支架的设计与制备：双重浸提技术。
J Biomed Mater Res A. 2021 Jun;109(6):981-993. doi: 10.1002/jbm.a.37087. Epub 2020 Sep 8.
9
In Vitro and In Vivo Evaluation of Poly (3-hydroxybutyrate)/Carbon Nanotubes Electrospun Scaffolds for Periodontal Ligament Tissue Engineering.聚（3-羟基丁酸酯）/碳纳米管电纺支架用于牙周韧带组织工程的体外和体内评价
J Dent (Shiraz). 2020 Mar;21(1):18-30. doi: 10.30476/DENTJODS.2019.77869.
10
Development and characterization of a PHB-HV-based 3D scaffold for a tissue engineering and cell-therapy combinatorial approach for spinal cord injury regeneration.用于组织工程和细胞治疗组合方法的脊髓损伤再生的基于 PHB-HV 的 3D 支架的开发和表征。
Macromol Biosci. 2013 Nov;13(11):1576-92. doi: 10.1002/mabi.201300178. Epub 2013 Aug 22.

引用本文的文献

1
Research Progress on the Synthesis, Modification, and Applications of Microbial Biopolymers.微生物生物聚合物的合成、改性及应用研究进展
Polymers (Basel). 2025 Jul 28;17(15):2053. doi: 10.3390/polym17152053.
2
Microbial Biopolymers: Trends in Synthesis, Modification, and Applications.微生物生物聚合物：合成、改性及应用趋势
Polymers (Basel). 2023 Mar 9;15(6):1364. doi: 10.3390/polym15061364.

本文引用的文献

1
Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells.PCL/nHAEA 纳米复合材料对牙髓干细胞成骨/成牙向分化的影响。
BMC Oral Health. 2022 Nov 16;22(1):505. doi: 10.1186/s12903-022-02527-1.
2
PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix.PAX9通过调节细胞外基质参与人诱导多能干细胞向牙周膜干细胞样分化。
Biomedicines. 2022 Sep 22;10(10):2366. doi: 10.3390/biomedicines10102366.
3
Nanomaterials in Scaffolds for Periodontal Tissue Engineering: Frontiers and Prospects.
用于牙周组织工程的支架中的纳米材料：前沿与展望
Bioengineering (Basel). 2022 Sep 1;9(9):431. doi: 10.3390/bioengineering9090431.
4
Novel approaches for periodontal tissue engineering.牙周组织工程的新方法。
Genesis. 2022 Sep;60(8-9):e23499. doi: 10.1002/dvg.23499. Epub 2022 Sep 10.
5
Biomaterials and biotechnology for periodontal tissue regeneration: Recent advances and perspectives.用于牙周组织再生的生物材料与生物技术：最新进展与展望
J Dent Res Dent Clin Dent Prospects. 2022 Winter;16(1):1-10. doi: 10.34172/joddd.2022.001. Epub 2022 May 29.
6
In vitro behaviour of human gingival fibroblasts cultured on 3D-printed titanium alloy with hydrogenated TiO nanotubes.在具有氢化 TiO 纳米管的 3D 打印钛合金上培养的人牙龈成纤维细胞的体外行为。
J Mater Sci Mater Med. 2022 Mar 2;33(3):27. doi: 10.1007/s10856-022-06649-4.
7
Periodontal Disease: The Good, The Bad, and The Unknown.牙周病：好的、坏的和未知的。
Front Cell Infect Microbiol. 2021 Dec 7;11:766944. doi: 10.3389/fcimb.2021.766944. eCollection 2021.
8
Current Concepts in the Management of Periodontitis.牙周炎治疗的现状。
Int Dent J. 2021 Dec;71(6):462-476. doi: 10.1111/idj.12630. Epub 2021 Feb 19.
9
Evaluation of polycaprolactone scaffold for guided bone regeneration in maxillary and mandibular defects: A clinical study.聚己内酯支架用于上颌和下颌骨缺损引导性骨再生的评估：一项临床研究。
Natl J Maxillofac Surg. 2020 Jul-Dec;11(2):207-212. doi: 10.4103/njms.NJMS_35_20. Epub 2020 Dec 16.
10
PHBV nanofibers promotes insulin-producing cells differentiation of human induced pluripotent stem cells.PHBV 纳米纤维促进人诱导多能干细胞向胰岛素分泌细胞的分化。
Gene. 2021 Feb 5;768:145333. doi: 10.1016/j.gene.2020.145333. Epub 2020 Dec 2.