Zarei Moein, Karbasi Saeed, Sari Aslani Fatemeh, Zare Shahrokh, Koohi-Hosseinabad Omid, Tanideh Nader
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Dept. of Biomaterials and Tissue Engineering, School of Advance Technology in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Dent (Shiraz). 2020 Mar;21(1):18-30. doi: 10.30476/DENTJODS.2019.77869.
Tissue engineering was an idea, today it has become a potential therapy for several tissues in dentistry, such as periodontal disease and oral mucosa.
In this experimental study, periodontal regeneration is one of the earliest clinical disciplines that has achieved therapeutic application in tissue engineering. The aim of the present study was to prepare electrospun Poly (3-hydroxybutyrate) (PHB)/1% Carbon nanotubes (CNTs) scaffolds for periodontal regeneration.
1% w/v of CNTs was added to the polymer solutions and electrospinned. Physical properties of the scaffolds were evaluated by scanning electron microscopy (SEM) and universal testing machine. Chemical characterization of the scaffolds was also assessed by Fourier-transform infrared spectroscopy (FTIR). Biological properties of the scaffolds were also evaluated in vitro by culturing periodontal ligament stem cells (PDLSCs) on the scaffolds for 10 days and in vivo by Implanting the scaffolds in rat model for 5 weeks.
Results showed that the scaffolds mimicked fibrous connective tissue of the (PDL). CNTs improved the mechanical properties, similar to 23-55 years old human PDL. In vitro biocompatibility study showed more attachment and proliferation of the PDLSCs for PHB/1%CNTs scaffolds compared to the PHB controls. In vivo study showed that CNTs in the scaffolds caused mild foreign body type giant cell reaction, moderate vascularization, and mild inflammation.
The results showed that the PHB/1%CNTs composite scaffolds might be potentially useful in periodontal regeneration.
组织工程曾经只是一个想法，如今它已成为治疗牙科多种组织（如牙周病和口腔黏膜）的一种潜在疗法。
在本实验研究中，牙周再生是最早在组织工程中实现治疗应用的临床学科之一。本研究的目的是制备用于牙周再生的电纺聚（3-羟基丁酸酯）（PHB）/1%碳纳米管（CNTs）支架。
将1%（w/v）的碳纳米管添加到聚合物溶液中并进行电纺。通过扫描电子显微镜（SEM）和万能试验机评估支架的物理性能。还通过傅里叶变换红外光谱（FTIR）对支架进行化学表征。通过在支架上培养牙周膜干细胞（PDLSCs）10天进行体外支架生物学性能评估，并通过将支架植入大鼠模型5周进行体内评估。
结果表明，支架模拟了牙周膜的纤维结缔组织。碳纳米管改善了力学性能，类似于23至55岁人类牙周膜的力学性能。体外生物相容性研究表明，与PHB对照组相比，PHB/1%碳纳米管支架上的牙周膜干细胞附着和增殖更多。体内研究表明，支架中的碳纳米管引起轻度异物型巨细胞反应、中度血管化和轻度炎症。
结果表明，PHB/1%碳纳米管复合支架可能对牙周再生具有潜在用途。
