Phillips Robert Kyle, Steiner Jörg M, Suchodolski Jan S, Lidbury Jonathan A
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.
Vet Sci. 2023 Jan 21;10(2):82. doi: 10.3390/vetsci10020082.
Isoprostanes are stable end products of lipid peroxidation that can be used as markers of oxidative stress. It was previously reported that a cohort of dogs with various liver diseases had increased urinary isoprostane concentrations compared to healthy control (HC) dogs. The aim of this study was to measure and report urinary isoprostane concentrations in dogs with different types of liver diseases. Urine was collected from 21 HC dogs and from 40 dogs with liver disease, including 25 with chronic hepatitis (CH), 7 with steroid hepatopathy (SH), and 8 with a congenital portosystemic shunt (CPSS). In this prospective, observational study, urinary 15-F-isoprostane (F-IsoP) concentrations were measured by liquid chromatography/mass spectrometry and normalized to urinary creatinine concentrations. Concentrations were compared between groups using a Kruskal-Wallis test followed by Dunn's multiple comparisons tests. Significance was set at < 0.05. The median (range) urinary F-IsoP to creatinine ratios (ng/mg UCr) were 3.6 (2.2-12.4) for HC dogs, 5.7 (2.4-11.3) for dogs with CH, 4.8 (2.4-8.6) for dogs with SH, and 12.5 (2.9-22.9) for dogs with CPSS. CPSS dogs had significantly higher urinary F-IsoP concentrations than HC dogs ( = 0.004), suggesting increased oxidative stress among this cohort.
异前列腺素是脂质过氧化的稳定终产物，可作为氧化应激的标志物。此前有报道称，与健康对照（HC）犬相比，一组患有各种肝脏疾病的犬尿中异前列腺素浓度升高。本研究的目的是测量并报告患有不同类型肝脏疾病的犬尿中异前列腺素的浓度。从21只HC犬和40只患有肝脏疾病的犬收集尿液，其中包括25只患有慢性肝炎（CH）的犬、7只患有类固醇性肝病（SH）的犬和8只患有先天性门体分流（CPSS）的犬。在这项前瞻性观察研究中，通过液相色谱/质谱法测量尿中15-F-异前列腺素（F-IsoP）的浓度，并将其标准化为尿肌酐浓度。使用Kruskal-Wallis检验，随后进行Dunn多重比较检验，比较各组之间的浓度。显著性设定为<0.05。HC犬尿F-IsoP与肌酐的中位数（范围）比值（ng/mg UCr）为3.6（2.2-12.4），CH犬为5.7（2.4-11.3），SH犬为4.8（2.4-8.6），CPSS犬为12.5（2.9-22.9）。CPSS犬尿F-IsoP浓度显著高于HC犬（=0.004），表明该组犬氧化应激增加。
