Heravi Amir S, Zhao Di, Michos Erin D, Doria De Vasconcellos Henrique, Ambale-Venkatesh Bharath, Lloyd-Jones Donald, Schreiner Pamela J, Reis Jared P, Shikany James M, Lewis Cora E, Ndumele Chiadi E, Guallar Eliseo, Ouyang Pamela, Hoogeveen Ron C, Lima Joao A C, Post Wendy S, Vaidya Dhananjay
School of Medicine, Johns Hopkins University, Baltimore, MD 21287, USA.
Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21287, USA.
Antioxidants (Basel). 2023 Feb 23;12(3):555. doi: 10.3390/antiox12030555.
Introduction-Oxidative stress is linked to cardiovascular diseases (CVD) and is suggested to vary by sex. However, few population-level studies have explored these associations and the majority comprise populations with advanced CVD. We assessed urinary isoprostane concentrations, a standard measure of oxidative stress, in a relatively young and healthy cohort, hypothesizing that higher oxidative stress is associated with an adverse cardiometabolic profile and female sex. Methods-Oxidative stress was measured in 475 women and 266 men, aged 48-55 years, from the Coronary Artery Risk Development in Young Adults (CARDIA) study using urinary 8-isoprostane (IsoP) and 2,3-dinor-8-isoprostane (IsoP-M). Multivariable-adjusted regression was used to evaluate cross-sectional associations. As secondary analysis, previously measured plasma F2-isoprostanes (plasma IsoP) from another CARDIA subset was similarly analyzed. Results-Mean (SD) ages for men and women were 52.1(2.3) and 52.2(2.2) years, respectively ( = 0.46), and 39% of the participants self-identified as Black (vs. White). Before adjustments, female sex was associated with higher median urinary IsoP (880 vs. 704 ng/g creatinine in men; < 0.01) and IsoP m (1675 vs. 1284 ng/g creatinine in men; < 0.01). Higher body mass index (BMI), high-density cholesterol (HDL-C), and triglycerides, current smoking, and less physical activity were associated with higher oxidative stress. Diabetes was not associated with urinary IsoP but was associated with lower IsoP m and plasma IsoP. Higher serum creatinine showed diverging associations with higher plasma and lower urinary isoprostane concentrations. Conclusions-Different isoprostane entities exhibit varying association patterns with CVD risk factors, and therefore are complementary, rather than interchangeable, in assessment of oxidative stress. Still, consistently higher isoprostanes among women, smokers, less active persons, and those with higher BMI and plasma triglycerides could reflect higher oxidative stress among these groups. While urinary isoprostanes are indexed to urinary creatinine due to variations in concentration, caution should be exercised when comparing groups with differing serum creatinine.
引言——氧化应激与心血管疾病（CVD）相关，且有研究表明其存在性别差异。然而，很少有基于人群水平的研究探讨这些关联，且大多数研究的对象为患有晚期心血管疾病的人群。我们在一个相对年轻且健康的队列中评估了尿中异前列腺素的浓度，这是氧化应激的一项标准指标，我们假设较高的氧化应激与不良的心脏代谢状况及女性性别相关。方法——采用尿8-异前列腺素（IsoP）和2,3-二硝基-8-异前列腺素（IsoP-M），对来自青年动脉粥样硬化风险发展研究（CARDIA）的475名年龄在48 - 55岁的女性和266名男性进行氧化应激测量。使用多变量调整回归来评估横断面关联。作为次要分析，对来自CARDIA另一亚组先前测量的血浆F2-异前列腺素（血浆IsoP）进行了类似分析。结果——男性和女性的平均（标准差）年龄分别为52.1（2.3）岁和52.2（2.2）岁（P = 0.46），39%的参与者自我认定为黑人（相对于白人）。在进行调整之前，女性性别与较高的尿IsoP中位数相关（男性为880 vs. 704 ng/g肌酐；P < 0.01）以及IsoP m（男性为1675 vs. 1284 ng/g肌酐；P < 0.01）。较高的体重指数（BMI）、高密度胆固醇（HDL-C）和甘油三酯、当前吸烟以及较少的身体活动与较高的氧化应激相关。糖尿病与尿IsoP无关，但与较低的IsoP m和血浆IsoP相关。较高的血清肌酐与较高的血浆异前列腺素浓度和较低的尿异前列腺素浓度呈现不同的关联。结论——不同的异前列腺素实体与心血管疾病风险因素呈现出不同的关联模式，因此在氧化应激评估中它们是互补的，而非可相互替代的。尽管如此，女性、吸烟者、活动较少者以及BMI和血浆甘油三酯较高者中异前列腺素持续较高可能反映了这些人群中较高的氧化应激。虽然由于浓度变化尿异前列腺素以尿肌酐为指标，但在比较血清肌酐不同的组时应谨慎。
