AOM 和 DSS 诱导的结肠炎相关性结直肠癌小鼠模型的方案。

Protocol for colitis-associated colorectal cancer murine model induced by AOM and DSS.

机构信息

School of Life Sciences, Institute for Immunology, MOE Key Laboratory of Protein Sciences, Center for Life Sciences, Advanced Innovation Center for Structural Biology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing Key Lab for Immunological Research on Chronic Diseases, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.

出版信息

STAR Protoc. 2023 Mar 17;4(1):102105. doi: 10.1016/j.xpro.2023.102105. Epub 2023 Feb 7.

DOI:10.1016/j.xpro.2023.102105

PMID:36853726

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9929628/

Abstract

Inflammatory bowel diseases (IBDs) contribute to the tumorigenesis of colorectal cancer (CRC). Here, we describe a step-by-step protocol for the construction of colitis-associated CRC murine model by sequential utilization of azoxymethane and dextran sulfate sodium. We also detail steps to determine the degree of murine intestinal inflammation and to generate colorectum Swiss roll for further histopathological analyses. This is a convenient and reproducible protocol for colitis-associated CRC murine model by the induction of general chemical reagents. For complete details on the use and execution of this protocol, please refer to Yang et al. (2022)..

摘要

炎症性肠病（IBD）可导致结直肠癌（CRC）的发生。在此，我们通过序贯利用氧化偶氮甲烷和葡聚糖硫酸钠，描述了一种构建结肠炎相关 CRC 小鼠模型的分步方案。我们还详细介绍了确定小鼠肠道炎症程度和生成结肠瑞士卷以进行进一步组织病理学分析的步骤。该方案通过诱导使用一般化学试剂，为结肠炎相关 CRC 小鼠模型的构建提供了一种便利且可重复的方法。如需了解该方案的详细使用和执行方法，请参考 Yang 等人（2022 年）的研究。