Department of Chemical and Systems Biology, Stanford University, USA.
Department of Developmental Biology, Stanford University, USA.
Curr Opin Genet Dev. 2023 Apr;79:102023. doi: 10.1016/j.gde.2023.102023. Epub 2023 Feb 26.
In the past two years, approaches relying on high-resolution microscopy and live-cell imaging have increasingly contributed to our understanding of the 3D genome organization and its importance for transcriptional control. Here, we describe recent progress that has highlighted how flexible and heterogeneous 3D chromatin structure is, on the length scales relevant to transcriptional control. We describe work that has investigated how robust transcriptional outcomes may be derived from such flexible organization without the need for clearly distinct structures in active and silent cells. We survey the latest state of the art in directly observing the dynamics of chromatin interactions, and suggest how some recent, apparently contradictory conclusions may be reconciled.
在过去的两年中，依赖高分辨率显微镜和活细胞成像的方法越来越有助于我们理解 3D 基因组组织及其对转录控制的重要性。在这里，我们描述了最近的进展，这些进展强调了在与转录控制相关的长度尺度上，3D 染色质结构是多么灵活和多样。我们描述了研究如何在不需要在活跃和沉默细胞中明确区分结构的情况下，从这种灵活的组织中获得稳健的转录结果的工作。我们调查了直接观察染色质相互作用动态的最新技术，并提出了如何调和一些最近看似矛盾的结论。
