School of Public Health, Suzhou Medical College of Soochow University, Suzhou, China; Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, Suzhou Medical College of Soochow University, Suzhou, China.
Department of Chronic Disease Control and Prevention, Changshu Center for Disease Control and Prevention, Suzhou, China.
J Nutr. 2023 Apr;153(4):1162-1169. doi: 10.1016/j.tjnut.2023.02.029. Epub 2023 Feb 26.
Previous studies suggested that β-alanine as a neurotransmitter could affect the pathogenesis of ischemic damage. However, the association between circulating β-alanine and risk of ischemic stroke (IS) has not been evaluated in populations.
We aimed to examine the association between β-alanine and IS risk in a nested case-control study.
We performed a case-control study nested within a prospective community-based cohort (n = 16457; median follow-up time: 5.3 y), which included 321 incident IS cases and 321 controls matched by age and sex. Β-alanine and other metabolites were measured in plasma after overnight fasting by LC-MS/MS. The association of β-alanine with risk of IS was evaluated by conditional logistic regression. BMI, current smoking, educational attainment, physical activity, total energy intake, family history of stroke, hypertension, diabetes, hyperlipidemia, and estimated GFR were adjusted in multivariable models.
There was a significant Spearman partial correlation between β-alanine and 4-pyridoxic acid (ρ = 0.239; P < 0.001). Participants with elevated β-alanine levels were more likely to develop IS with an adjusted OR of 1.26 (95% CI: 1.06-1.51; P = 0.011) (per standard deviation increment). This association remained significant after excluding the first 2 y of follow-up, and after further adjustment for red meat intake, total protein intake, medication use, or vitamin B6 indicators.
Our novel findings revealed that plasma β-alanine at baseline were positively associated with risk of IS and may function as an early biomarker of IS risk.
先前的研究表明，β-丙氨酸作为一种神经递质可能影响缺血性损伤的发病机制。然而，在人群中尚未评估循环β-丙氨酸与缺血性脑卒中（IS）风险之间的关联。
我们旨在通过巢式病例对照研究检验β-丙氨酸与 IS 风险之间的关联。
我们进行了一项嵌套于前瞻性社区队列研究中的病例对照研究（n = 16457；中位随访时间：5.3 年），其中包括 321 例新发 IS 病例和 321 例年龄和性别匹配的对照者。通过 LC-MS/MS 法在隔夜禁食后测定血浆中的β-丙氨酸和其他代谢物。通过条件 logistic 回归评估β-丙氨酸与 IS 风险的关联。在多变量模型中调整 BMI、当前吸烟状况、教育程度、身体活动、总能量摄入、卒中家族史、高血压、糖尿病、高脂血症和估计肾小球滤过率。
β-丙氨酸与 4-吡啶酸之间存在显著的 Spearman 偏相关（ρ = 0.239；P < 0.001）。β-丙氨酸水平升高的参与者发生 IS 的可能性更高，调整后的 OR 为 1.26（95%CI：1.06-1.51；P = 0.011）（每标准偏差递增）。排除随访的前 2 年后，该关联仍然显著，且进一步调整红肉摄入量、总蛋白摄入量、药物使用或维生素 B6 指标后，该关联仍然显著。
我们的新发现表明，基线时的血浆β-丙氨酸与 IS 风险呈正相关，可能作为 IS 风险的早期生物标志物。
