Department of Microbiology, National Health Laboratory Service, KwaZulu-Natal Academic Complex, Inkosi Albert Luthuli Central Hospital, Durban, South Africa.
Department of Medical Microbiology, School of Laboratory Medicine and Medical Science, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
Breastfeed Med. 2023 Apr;18(4):265-271. doi: 10.1089/bfm.2022.0215. Epub 2023 Feb 28.
Pasteurized donor human milk provides nutrition and bioactive factors for infant growth and health when a mother's own milk is not available. Bacteriological testing is recommended for each pasteurized batch of donor milk before distribution to ensure that the milk is safe for use. Charm Peel Plates (CPPs) are a simplified, easy-to-use culture method for detecting microorganisms in milk and milk products. This study investigates the feasibility of using CPPs as an alternative test for current standard postpasteurization screening by human milk banks (HMBs), particularly those in resource-limited settings. The aim of this study was to evaluate the feasibility of using the CPP versus the 5% horse blood agar (HBA) plate (standard South African National Health Laboratory Service method) for detecting bacterial growth in pasteurized human milk samples. For each of the 50 pasteurized donor milk samples, 100-μL aliquots were cultured on routine HBA and 1 mL on CPPs for the total bacterial colony count. Any positive growth was identified using VITEK 2 (bioMérieux). To demonstrate the ability of CPPs to support bacterial growth, four spiked samples were tested. Concurrent negative test results were reported for 49/50 (98%) samples with only one positive test with HBA. The CPP is equivalent to HBA for detection of bacterial growth. Additional advantages of CPPs are ease of use and cost-effectiveness. The CPP is therefore recommended as a point-of-care, bacteriological screening method for donor human milk by HMBs, particularly those in resource-limited settings.
巴氏消毒后的捐赠人母乳为无法获得母亲自身母乳的婴儿提供生长和健康所需的营养和生物活性因子。在分发之前，建议对每批巴氏消毒后的捐赠人母乳进行细菌学检测，以确保母乳可以安全使用。魅力削皮盘（CPPs）是一种简化易用的检测牛奶和奶制品中微生物的培养方法。本研究探讨了 CPPs 作为人乳库（HMB）当前巴氏消毒后筛选的替代检测方法的可行性，特别是在资源有限的环境中。本研究的目的是评估使用 CPP 替代 5%马血琼脂（HBA）平板（南非国家卫生实验室服务标准方法）检测巴氏消毒人乳样本中细菌生长的可行性。对于 50 个巴氏消毒的捐赠人乳样本中的每一个，用 100μL 等分试样在常规 HBA 上培养，用 1mL 在 CPP 上培养进行总细菌菌落计数。使用 VITEK 2（生物梅里埃）对任何阳性生长进行鉴定。为了证明 CPP 能够支持细菌生长，对四个加标样本进行了测试。49/50（98%）个样本的 CPP 和 HBA 检测结果均为阴性，仅 1 个样本的 HBA 检测结果为阳性。CPP 与 HBA 一样可用于检测细菌生长。CPP 的额外优点是易于使用和具有成本效益。因此，CPP 被推荐为 HMB，特别是资源有限环境中的 HMB，用于捐赠人母乳的即时、现场细菌学筛查方法。
