Zhu Afang, Ali Shujat, Jiao Tianhui, Wang Zhen, Ouyang Qin, Chen Quansheng
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, P. R. China.
College of Electrical and Electronic Engineering, Wenzhou University, Wenzhou, P. R. China.
Compr Rev Food Sci Food Saf. 2023 May;22(3):1466-1494. doi: 10.1111/1541-4337.13118. Epub 2023 Mar 1.
Rapid control and prevention of diseases caused by foodborne pathogens is one of the existing food safety regulatory issues faced by various countries and has received wide attention from all sectors of society. The development of rapid and reliable detection methods for foodborne pathogens remains a hot research area for food safety and public health because of the limitations of complex steps, time-consuming, low sensitivity, or poor selectivity of commonly used methods. Surface-enhanced Raman spectroscopy (SERS), as a novel spectroscopic technique, has the advantages of high sensitivity, selectivity, rapid and nondestructive detection and has exhibited broad application prospects in the determination of pathogenic bacteria. In this study, the enhancement mechanisms of SERS are briefly introduced, then the characteristics and properties of liquid-phase, rigid solid-phase, and flexible solid-phase are categorized. Furthermore, a comprehensive review of the advances in label-free or label-based SERS strategies and SERS-compatible techniques for the detection of foodborne pathogens is provided, and the advantages and disadvantages of these methods are reviewed. Finally, the current challenges of SERS technology applied in practical applications are listed, and the possible development trends of SERS in the field of foodborne pathogens detection in the future are discussed.
快速控制和预防食源性病原体引起的疾病是各国目前面临的食品安全监管问题之一，受到了社会各界的广泛关注。由于常用方法存在步骤复杂、耗时、灵敏度低或选择性差等局限性，食源性病原体快速可靠检测方法的开发仍是食品安全和公共卫生领域的一个热门研究方向。表面增强拉曼光谱（SERS）作为一种新型光谱技术，具有灵敏度高、选择性好、检测快速且无损等优点，在病原菌检测方面展现出广阔的应用前景。本研究简要介绍了SERS的增强机制，然后对液相、刚性固相和柔性固相的特性进行了分类。此外，对用于检测食源性病原体的无标记或基于标记的SERS策略以及与SERS兼容的技术进展进行了全面综述，并对这些方法的优缺点进行了评述。最后，列出了SERS技术在实际应用中面临的当前挑战，并讨论了SERS在未来食源性病原体检测领域可能的发展趋势。
