School of Chemistry and Materials Science, Jiangsu Normal University, 221116, Xuzhou, People's Republic of China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, People's Republic of China.
Mikrochim Acta. 2021 May 27;188(6):201. doi: 10.1007/s00604-021-04864-4.
The incidence of disease arising from food-borne pathogens is increasing continuously and has become a global public health problem. Rapid and accurate identification of food-borne pathogens is essential for adopting disease intervention strategies and controlling the spread of epidemics. Surface-enhanced Raman spectroscopy (SERS) has attracted increasing interest due to the attractive features including simplicity, rapid measurement, and high sensitivity. It can be used for rapid in situ sensing of single and multicomponent samples within the nanostructure-based confined space by providing molecular fingerprint information and has been demonstrated to be an effective detection strategy for pathogens. This article aims to review the application of SERS to the rapid sensing of food-borne pathogens in food matrices. The mechanisms and advantages of SERS, and detection strategies are briefly discussed. The latest progress on the use of SERS for rapid detection of food-borne bacteria and viruses is considered, including both the labeled and label-free detection strategies. In closing, according to the current situation regarding detection of food-borne pathogens, the review highlights the challenges faced by SERS and the prospects for new applications in food safety. Graphical abstract In this review, the advances on the SERS detection of pathogens over the past decades have been reviewed, focusing on the improvements in sensitivity, reproducibility, specificity, and the performance of the SERS-based assay in complex analytical scenarios.
食源性病原体引起的疾病发病率不断上升，已成为全球公共卫生问题。快速准确地识别食源性病原体对于采取疾病干预策略和控制疫情传播至关重要。表面增强拉曼光谱（SERS）因其具有简单、快速测量和高灵敏度等特点，引起了越来越多的关注。它可以通过提供分子指纹信息，在基于纳米结构的受限空间内对单一组分和多组分样品进行快速原位传感，已被证明是一种有效的病原体检测策略。本文旨在综述 SERS 在食品基质中快速检测食源性病原体方面的应用。简要讨论了 SERS 的机制和优点以及检测策略。考虑了 SERS 用于快速检测食源性细菌和病毒的最新进展，包括标记和无标记检测策略。最后，根据食源性病原体检测的现状，本文综述了 SERS 面临的挑战以及在食品安全领域新应用的前景。
