Wu Hui, Meng Zexuan, Wang Jian, Yao Guoqing, Yang Lu, Zeng Zhongyuan, She Kepeng, Zhao Shuang, Wang Guixue, Zhang Yuchan, Zang Guangchao
Institute of Life Science, Laboratory of Tissue and Cell Biology, Lab Teaching & Management Center, Chongqing Medical University, Chongqing, 400016, China.
Institute of Life Science, Laboratory of Tissue and Cell Biology, Lab Teaching & Management Center, Chongqing Medical University, Chongqing, 400016, China; Department of Pathophysiology, Chongqing Medical University, Chongqing, China.
Biosens Bioelectron. 2023 May 1;227:115149. doi: 10.1016/j.bios.2023.115149. Epub 2023 Feb 13.
Accurate dopamine (DA) monitoring with high stability is essential for investigating the chemical basis of brain function and pathology. Electrochemical-based tissue-implantable carbon fiber electrodes (CFEs) show great potential in sensing the dynamics of neurochemicals at a sub-second timescale. However, their anti-fouling property, selectivity, and stability pose challenges. Here, we presented a novel strategy to enhance electrode biocompatibility and stability by modifying CFE with a chitosan (CS) film, brain cell membrane (M), and aptamer cholesterol amphiphiles (DNA-cho). We found that CFE was uniformly covered by a cicada-like membrane after being modified. Electrochemical characterizations indicated that DNA-cho-M-CS-CFE exhibited a wide linear range of DA concentration and showed high sensitivity, specificity, and stability. The electrode also presented excellent fouling resistance and biocompatibility. Moreover, the biosensor was used to detect DA in K-induced brain slices and PC12 cells with a satisfactory stability and sensitivity and to prove that LPS treatment leads to the delayed and decreased release of DA. DNA-cho-M-CS-CFE showed excellent electrochemical performance and unique advantages for long-term in vivo sensing of living cells, thus providing a new feasible scheme for studying neurochemical kinetics and brain diseases.
高稳定性的精确多巴胺（DA）监测对于研究脑功能和病理学的化学基础至关重要。基于电化学的可植入组织的碳纤维电极（CFE）在亚秒级时间尺度上检测神经化学物质的动态变化方面显示出巨大潜力。然而，它们的抗污性能、选择性和稳定性带来了挑战。在此，我们提出了一种新策略，通过用壳聚糖（CS）膜、脑细胞膜（M）和适配体胆固醇两亲物（DNA-cho）修饰CFE来增强电极的生物相容性和稳定性。我们发现修饰后的CFE被一层蝉翼状的膜均匀覆盖。电化学表征表明，DNA-cho-M-CS-CFE对DA浓度具有宽线性范围，且表现出高灵敏度、特异性和稳定性。该电极还具有出色的抗污性和生物相容性。此外，该生物传感器用于检测钾诱导的脑切片和PC12细胞中的DA，具有令人满意的稳定性和灵敏度，并证明脂多糖处理导致DA释放延迟和减少。DNA-cho-M-CS-CFE表现出优异的电化学性能和在活细胞长期体内传感方面的独特优势，从而为研究神经化学动力学和脑部疾病提供了一种新的可行方案。
