Department of Clinical Nutrition, School of Nutrition & Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Pediatric Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Pediatr. 2023 Mar 1;23(1):95. doi: 10.1186/s12887-023-03867-9.
The widespread presence of childhood obesity has increased considerably over three decades. The present study was designed to investigate expression patterns of miR-146a, miR-155, miR-15a, miR-193a, and miR-122 in peripheral blood mononuclear cells (PBMCs) in children who are obese along with their association with metabolic and inflammatory biomarkers.
Ninety test subjects were admitted. The profile of blood pressure, resting energy expenditure (REE), anthropometric measures, body composition, dietary intakes, physical activity levels, insulin, and lipid profile, fasting blood glucose (FBG), high-sensitivity C-reactive protein (hs-CRP), and pubertal stage have been measured. Total RNA (including small RNAs) was extracted from PBMCs. The expression levels of miRNAs were measured by stem-loop RT-qPCR.
The miR-155a expression level was significantly lower in obese children, children with high hs-CRP, and children with high-fat mass. Obese girls had significantly higher PBMC levels of miR-122. MiR-155a had a significant negative association with fasting insulin, HOMA-IR, and hs-CRP. There were significant positive associations between miR-193a and miR-122 expression levels and fasting insulin, HOMA-IR, and TG. MiR-15a was positively correlated with fasting insulin and HOMA-IR. Children with metabolic syndrome, insulin resistance, and high-fat mass had higher PBMC levels of miR-122 and miR-193a. Higher miR-193a and miR-122 levels were also detected in PBMCs of children with fast REE, compared to those with slow REE, and the subjects with high hs-CRP, respectively.
lower level of miR-155 expression in obese subjects and significant associations unfolds the need for more studies to detect the possible underlying mechanisms.
儿童肥胖的广泛存在在过去三十年中大大增加。本研究旨在研究肥胖儿童外周血单个核细胞（PBMC）中 miR-146a、miR-155、miR-15a、miR-193a 和 miR-122 的表达模式及其与代谢和炎症生物标志物的关系。
共纳入 90 名受试者。测量了血压、静息能量消耗（REE）、人体测量学指标、身体成分、饮食摄入、体力活动水平、胰岛素和血脂谱、空腹血糖（FBG）、高敏 C 反应蛋白（hs-CRP）和青春期阶段。从 PBMC 中提取总 RNA（包括小 RNA）。采用茎环 RT-qPCR 法测定 miRNA 的表达水平。
肥胖儿童、hs-CRP 高的儿童和高脂肪量的儿童 miR-155a 表达水平明显降低。肥胖女孩 PBMC 中的 miR-122 水平明显升高。miR-155a 与空腹胰岛素、HOMA-IR 和 hs-CRP 呈显著负相关。miR-193a 和 miR-122 表达水平与空腹胰岛素、HOMA-IR 和 TG 呈显著正相关。miR-15a 与空腹胰岛素和 HOMA-IR 呈正相关。患有代谢综合征、胰岛素抵抗和高脂肪量的儿童 PBMC 中的 miR-122 和 miR-193a 水平较高。与 REE 较慢的儿童相比，快速 REE 的儿童和 hs-CRP 较高的儿童的 PBMC 中 miR-193a 和 miR-122 水平也较高。
肥胖患者 miR-155 表达水平较低，且存在显著相关性，这表明需要进一步研究以检测可能的潜在机制。
