Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, College of Life Sciences, Tianjin Normal University, Tianjin, China.
PeerJ. 2023 Feb 24;11:e14897. doi: 10.7717/peerj.14897. eCollection 2023.
Reintroduction is an effective strategy in the conservation of endangered species under scientific monitoring. Intestinal flora plays an important role in the envir onmental adaptation of endangered Père David's deer (). In this study, 34 fecal samples from were collected from different habitats in Tianjin city of China to investigate differences in the intestinal flora under captive and semi-free-ranging conditions. Based on 16S rRNA high-throughput sequencing technology, a total of 23 phyla and 518 genera were obtained. Firmicutes was dominant in all individuals. At the genus level, (13.05%) and (8.94%) were dominant in captive individuals, while (26.53%) and (11.33%) were dominant in semi-free-ranging individuals. Alpha diversity results showed that the intestinal flora richness and diversity were significantly ( < 0.001) higher in captive individuals than in semi-free-ranging individuals. Beta diversity analysis also showed a significant difference ( = 0.001) between the two groups. In addition, some age- and sex-related genera such as were identified. In summary, the structure and diversity of intestinal flora showed significant habitat variation. This is the first time an analysis has been undertaken of the structural differences of the intestinal flora in Père David's deer, under different habitats in the warm temperate zone, providing a reference basis for the conservation of endangered species.
重引入是濒危物种在科学监测下保护的有效策略。肠道菌群在濒危麋鹿（）的环境适应中起着重要作用。本研究从中国天津市的不同生境中采集了 34 份粪便样本，以调查圈养和半散养条件下肠道菌群的差异。基于 16S rRNA 高通量测序技术，共获得 23 个门和 518 个属。厚壁菌门在所有个体中均占优势。在属水平上，（13.05%）和（8.94%）在圈养个体中占优势，而（26.53%）和（11.33%）在半散养个体中占优势。α多样性结果表明，圈养个体的肠道菌群丰富度和多样性明显（<0.001）高于半散养个体。β多样性分析也显示两组之间存在显著差异（=0.001）。此外，还鉴定出一些与年龄和性别相关的属，如。总之，肠道菌群的结构和多样性表现出明显的栖息地变化。这是首次对温带地区不同生境下麋鹿肠道菌群结构差异进行分析，为濒危物种保护提供了参考依据。
