Wu Hong, Wu Fang-Ting, Zhou Qi-Hai, Zhao Da-Peng
Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, College of Life Sciences, Tianjin Normal University, Tianjin, China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, China.
Front Microbiol. 2021 Mar 25;12:649466. doi: 10.3389/fmicb.2021.649466. eCollection 2021.
The oriental white stork () is considered an endangered species based on the International Union for Conservation of Nature (IUCN) Red List. This study presents the first evidence on comparative analysis of gut microbial diversity of from various breeding conditions. To determine the species composition and community structure of the gut microbiota, 24 fecal samples from Tianjin Zoo and Tianjin Qilihai Wetland were characterized by sequencing 16S rRNA gene amplicons using the Illumina MiSeq platform. Firmicutes was found to be the predominant phylum. Analysis of community structure revealed significant differences in the species diversity and richness between the populations of the two breeding conditions. The greatest α-diversity was found in wild , while artificial breeding storks from Tianjin Zoo had the least α-diversity. Principal coordinates analysis showed that the microbial communities were different between the two studied groups. In conclusion, this study reveals the species composition and structure of the gut microbiota of oriental white storks under two breeding conditions, and our findings could contribute to the integrative conservation of this endangered bird.
根据国际自然保护联盟（IUCN）红色名录，东方白鹳（ ）被视为濒危物种。本研究首次提供了不同繁殖条件下东方白鹳肠道微生物多样性比较分析的证据。为确定肠道微生物群的物种组成和群落结构，利用Illumina MiSeq平台对天津动物园和天津七里海湿地的24份粪便样本进行16S rRNA基因扩增子测序分析。结果发现厚壁菌门是主要菌门。群落结构分析表明，两种繁殖条件下东方白鹳种群的物种多样性和丰富度存在显著差异。野生东方白鹳的α多样性最高，而天津动物园的人工繁育东方白鹳α多样性最低。主坐标分析表明，两个研究组的微生物群落不同。总之，本研究揭示了两种繁殖条件下东方白鹳肠道微生物群的物种组成和结构，我们的研究结果有助于对这种濒危鸟类进行综合保护。
